Pteridic acid hydrate and pteridic acid C produced by Streptomyces pseudoverticillus YN17707 induce cell cycle arrest  

作　　者：HAN Bing LI Wen-Xin CUI Cheng-Bin 

机构地区：[1]Beijing Institute of Biomedicine [2]Beijing Institute of Pharmacology and Toxicology

出　　处：《Chinese Journal of Natural Medicines》2015年第6期467-470,共4页中国天然药物（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.30472079);National Basic Research Program of China(No.2006CB504100)

摘　　要：The present study aimed at identifying cell cycle inhibitors from the fermentation broth of Streptomyces pseudoverticillus YN17707. Activity-guided isolation was performed on ts FT210 cells. Compounds were isolated through various chromatographic methods and elucidated by spectroscopic analyses. Flow cytometry was used to evaluate the cell cycle inhibitory activities of the fractions and compounds. Two compounds were obtained and identified as pteridic acid hydrate(1) and pteridic acid C(2), which arrested the ts FT210 cells at the G0/G1 phase with the MIC values being 32.8 and 68.9 μmol·L-1, respectively. These results provide a basis for future development of Compounds 1 and 2 as novel cell cycle inhibitors for cancer therapy.The present study aimed at identifying cell cycle inhibitors from the fermentation broth of Streptomyces pseudoverticillus YN17707. Activity-guided isolation was performed on tsFT210 cells. Compounds were isolated through various chromatographic methods and elucidated by spectroscopic analyses. Flow cytometry was used to evaluate the cell cycle inhibitory activities of the fractions and compounds. Two compounds were obtained and identified as pteridic acid hydrate （1） and pteridic acid C （2）, which arrested the tsFT210 cells at the G0/G1 phase with the MIC values being 32.8 and 68.9 μmol·L-1, respectively. These results provide a basis for future development of Compounds 1 and 2 as novel cell cycle inhibitors for cancer therapy.

关 键 词：Cell cycle inhibitor Streptomyces pseudoverticillus Spirocyclic polyketide Pteridic acid Flow cytometry 

分 类 号：Q936[生物学—微生物学] R284.1[医药卫生—中药学]