蕙兰(Cymbidium faberi Rolfe)基因组大小测定  

Estimation of Genome Size of Cymbidium faberi Rolfe

作　　者：汪琛颖[1,2] 张坤[3] 梁芳[4] 崔波[4] 马润林[5] 

机构地区：[1]郑州师范学院生命科学学院,河南郑州450044 [2]郑州师范学院分子生物学实验室,河南郑州450044 [3]黑龙江大学生命科学学院,黑龙江哈尔滨150080 [4]郑州师范学院生物工程研究所,河南郑州450044 [5]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《北方园艺》2015年第11期86-90,共5页Northern Horticulture

基　　金：河南省科技计划资助项目(132300410358)

摘　　要：以蕙兰为试材,采用流式细胞分析技术,以铁皮石斛为外参,对其基因组大小进行估算。结果表明:蕙兰的基因组大小为(4.39±0.12)Gb,即2CDNA含量为8.98pg。该研究探索了用流式细胞仪分析蕙兰基因组大小的方法,所得数据为蕙兰基因组测序提供了必要的基础。Taking Cymbidium faberi as material, using D. officinale Kimura et Migo as an external reference, flow cytometry measurements were performed when the genome size of Cymbidium faberi was estimated. The results showed that,the estimated genome size of C. faberi was （4. 39±0. 12）Gb,or the content of 2C DNA was about 8. 98 pg. The present study described a modified method of genome size analysis by using flow cytometry in C. faberi. The data available of DNA content obtained in this study was helpful to the future genome sequencing research.

关 键 词：蕙兰 基因组大小 流式细胞术 铁皮石斛 

分 类 号：S682.31[农业科学—观赏园艺]