铁皮石斛菌根的建立及相关基因的表达研究  

Establishment of Dendrobium officinale Mycorrhiza System and the Study on Its Related Gene Expression

作　　者：冒佩佩 周丽婷[1] 吴琳[1] 吴剑丙[1] 王慧中[1] 

机构地区：[1]杭州师范大学生命与环境科学学院,浙江省药用植物种质改良与质量控制技术重点实验室,浙江杭州310036

出　　处：《北方园艺》2015年第11期98-102,共5页Northern Horticulture

基　　金：浙江省自然科学基金资助项目(LY14C010003);杭州市科技发展计划资助项目(20130432B07);浙江省大学生科技创新活动计划暨新苗人才计划资助项目(ZX13005002070)

摘　　要：围绕菌根共生体系中菌根真菌与宿主植物的互作机制展开研究,以石斛小菇Mycena dendrobii与铁皮石斛Dendrobium officinale为试验材料,建立二者的共生体系,对其根系切片进行显微观察,并采用实时荧光定量PCR技术检测石斛基因的表达情况。结果表明:二者能成功形成菌根结构,分支酸合成酶基因和甘露糖特异结合植物血凝素基因在共生早期发生上调,参与了共生的调节。试验旨在为今后阐明菌根真菌与兰科植物的共生机制奠定理论基础。The study focused on the interaction mechanisms between mycorrhizal fungi and host plants. ＂Faking Mycena dendrobii and Dendrobium officinale as materials, the symbiotic system of M. dendrobii and D. officinale was constructed. The microscopic observation of microsection of symbiont showed that M. dendrobii-D, officinale mycorrhizal symbiont was established successfully. Simultaneously, the chorismate synthase gene and mannose-specific lectin gene were tested using fluorescent quantitative Polymerase Chain Reaction, showing that these genes were up-regulated in early symbiosis stage. The results showed that these genes participate in the regulation of symbiosis. This study laid the foundation for clarifying the interaction molecular mechanisms between orchid and mycorrhizal fungi.

关 键 词：铁皮石斛 石斛小菇 共生 差异表达 

分 类 号：S682.31[农业科学—观赏园艺]