响应面法优化野火球总黄酮微波提取工艺  

The Determination and Extraction Technology Optimization of Flavonoids Content fromTrifolium lupinaster L.

作　　者：杜娟[1] 吴岚[1] 闫红[1] 李来军[1] 陈宁[1] 张楠楠[1] 

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007

出　　处：《北方园艺》2015年第11期129-132,共4页Northern Horticulture

基　　金：黑龙江省自然科学基金资助项目(D201209);黑龙江省教育厅科研资助项目(12531714)

摘　　要：以野火球为试材,选择乙醇体积分数、微波时间、微波功率、液料比4个因素,利用微波提取技术对野火球全草总黄酮成分提取工艺进行研究。通过Box-Behnken中心组合设计试验和响应面分析法,模拟得到二次多项式回归方程的预测模型。结果表明:最佳提取工艺条件为乙醇浓度20%,微波时间45s,微波功率350W,液料比1∶20mL/g。为野火球的开发利用提供参考依据和科学数据。Taking TriJblium lupinaster as material, the effects of ethanol volume fraction, microwave irradiation time, microwave power,material liquid ratio on the extraction rate of total flavonoids content were studied based on the single factor experiment using Box-Behnken central composite experimental design and response surface analysis （RSM） method,established two regression equations of microwave extraction of flavonoids, and the extraction rate of total flavonoids content by response surface graph drawing and the contour map as the response value. The results showed that,through the analysis to determine the best extraction conditions test response surface, that were 20% ethanol concentration,microwave time 45 seconds,microwave power 350 W,the ratio of liquid to solid 1 ： 20 mL/g. It provided a theoretical basis and technical support for further development and utilization of Trifolium lupinaster.

关 键 词：野火球 总黄酮成分 微波提取法 响应面分析 

分 类 号：S567.239[农业科学—中草药栽培]