在不同组织中淀粉样物质5种染色法的比较  

In different tissues of amyloid 5 staining methods comparison

作　　者：涂贵兰[1] 文安智[1] 

机构地区：[1]贵州省人民医院病理科,贵阳550002

出　　处：《临床与病理杂志》2015年第5期740-745,共6页Journal of Clinical and Pathological Research

摘　　要：目的:探寻淀粉样物质更好的特殊染色方法。方法:采用甲醇刚果红、甲基紫、Bennhola氏刚果红、Freudenthal氏刚果红、碱性刚果红5种染色,显微镜下观察组织切片中淀粉样蛋白的染色情况。结果:喉部组织、皮肤组织、肾穿刺组织经甲醇刚果红、甲基紫、Bennhola氏刚果红、Freudenthal氏刚果红、碱性刚果红染色方法分别将淀粉样物质均染成红色但其鲜艳程度不同,背景着色程度不同。结论:喉部组织、皮肤组织、肾穿刺组织在淀粉样物质选择特殊染色方法时甲醇刚果红是阳性着色部位最准确、色彩最鲜明、背景最干净的方法。Objective：To explore the method of special staining of amyloid better. Methods：Using methanol Congo red, methyl violet, Bennhola’s Freudenthal’s Congo red, Congo red, alkaline Congo red those ifve staining, staining to detect amyloid protein in tissue sections under microscope. Results：The larynx tissue, skin tissue, renal biopsy by methanol Congo red, methyl violet, Bennhola’s Freudenthal’s Congo red, Congo red, alkaline Congo red respectively amyloid are dyed red but its bright degree is different, color the background of different degree. Conclusion：Laryngeal tissue, skin tissue, kidney tissue in the amyloid selection of special staining method of methanol is part of Congo red positive staining the most accurate and most distinctive background color, the cleanest method.

关 键 词：淀粉样物质 喉部组织 皮肤组织 肾穿刺组织 特殊染色 

分 类 号：R446.8[医药卫生—诊断学]