基于“生消熟补”理论的三七补血作用及其机理研究  

Study on the effect of bloodenriching and mechanism of steamed notog1inseng powder based on the theory of“prepared notoginseng can enrich the Blood and Qi while the raw one do the opposite”

作　　者：何宜航 桑文涛 杨桂燕[1] 邹俊波[1] 阳向波 纪奇森 杨明[1,3] 

机构地区：[1]成都中医药大学药学院,成都611137 [2]四川联成迅康医药股份有限公司,成都610000 [3]江西中医药大学制剂教育部重点实验室,南昌330004

出　　处：《世界中医药》2015年第5期647-651,共5页World Chinese Medicine

摘　　要：目的:对比生、熟三七粉对环磷酰胺所致血虚小鼠的治疗作用,并研究其机理。方法:108只KM小鼠按体重随机分为空白组,模型组,阳性组,生、熟三七低、中、高剂量组,每组12只。其中生、熟三七粉按低、中、高剂量(585 mg·kg-1,1170 mg·kg-1,2340 mg·kg-1)、阳性组以8 m L·kg-1剂量灌胃复方阿胶浆,连续12 d,自给药起前3 d以70 mg·kg-1剂量连续腹腔注射环磷酰胺生理盐水溶液制作血虚模型,试验结束后以外周WBC、RBC、Hb、PLT及脏器指数(脾脏、胸腺)为指标对生、熟三七补血作用进行比较;并以流式细胞技术测定骨髓DNA含量及骨髓细胞CD34+的表达,结合CFU-GM、BFU-E、CFU-E、CFU-Meg的培养研究熟三七的补血机制。结果:与空白组相比,模型组小鼠外周WBC、RBC、HB及PLT、脏器指数均有极显著下降(P<0.01),骨髓DNA含量及CD34+均显著下降(P<0.01);与模型组相比,熟三七高、中、低剂量组均能显著升高外周WBC、RBC、Hb、PLT(P<0.05),并均能显著提高脏器指数(胸腺、脾脏)(P<0.05),同等剂量生三七则无明显效果;与模型组相比,熟三七组骨髓DNA及细胞CD34+表达率均有显著升高(P<0.05),CFU-GM、BFU-E、CFUE、CFU-Meg集落数均有显著增加(P<0.05)。结论:熟三七可通过促进骨髓细胞增殖而达到补血的功效。Objective：To compare the different effect between raw and steamed panax notoginseng powder on deficiency of blood caused by cyclophosphamide , and its mechanism.Methods：A total of 108 KM mice were randomly divided by weight into：nor-mal group, model group, positive group, low, medium and high dose group of raw and steamed panax notoginseng powder ,12 mice in each group .The raw and steamed groups were given three different dose of panax notoginseng powder （ 585 ,1170 ,2340 mg·kg -1 ） , the positive group was given with 8 mL· kg-1 Compound E-Jiao slurry ,lavage for 12 days.The deficiency of blood model were made in the first three days by intraperitoneal injection of cyclophosphamide with the concentration of 70 mg·kg-1 in mice.After the experiment , we evaluated the effectiveness of drugs by WBC , RBC, Hb, PLT and organ index （ spleen and thy-mus） .Then flow cytometry was used to measure DNA content of bone marrow cells and CD 34 ＋expression, conbined with the cul-ture of CFU-GM, BFU-E, CFU-E, CFU-Meg to research the blood enriching function of steamed panax notoginseng powder .Re-sults：Compared with the normal group , the WBC, RBC, Hb , PLT and the organ index （ spleen and thymus ） of model group were significantly decreased （ P〈0.01 ） , DNA content of bone marrow cells and CD 34 ＋expression were significantly decreased （P〈0.01）;Compared with model group , the high, medium and low dose group of steamed panax notoginseng powder were all significantly increased by WBC , RBC, Hb, PLT （ P〈0.05 ） , and can significantly increase the organs index thymus and spleen （ P〈0.05 ） , the same dose of raw panax notoginseng had no obvious effect;Compared with model group , the DNA content of bone marrow cells and CD34 ＋expression of steamed panax notoginseng were significantly higher （P〈0.05）, CFU-GM, BFU-E, CFU-E, CFU-Meg colony forming units were significantly increased too （P〈0.05）.Conclusion：Steamed panax notoginseng can exert the blood enriching function b

关 键 词：熟三七 环磷酰胺 再障血虚 骨髓 补血 

分 类 号：R283.3[医药卫生—中药学]