刺五加注射液在正常大鼠与脑缺血-再灌注损伤疾病大鼠体内药动学比较  

Comparing pharmacokinetics of Acanthopanax Injection in normal and cerebral ischemia-reperfusion rats

作　　者：范惠霞[1,2,3] 邓志鹏[1,3] 王福文[1] 徐晓婷[1,2,3] 姚庆强[1,3] 

机构地区：[1]山东省医学科学院药物研究所,山东济南250062 [2]济南大学山东省医学科学院医学与生命科学学院,山东济南250022 [3]山东省罕少见病重点实验室,山东济南250062

出　　处：《中成药》2015年第6期1215-1221,共7页Chinese Traditional Patent Medicine

摘　　要：目的比较刺五加注射液(紫丁香苷、刺五加苷E、异嗪皮啶等)在正常大鼠与脑缺血-再灌注损伤大鼠体内药代动力学行为。方法采用longa法建立大鼠局灶性脑缺血-再灌注损伤疾病模型,采用液相色谱-串联四级杆质谱法测定不同时间点大鼠血浆中紫丁香苷、刺五加苷E及异嗪皮啶的含有量,用药代动力学软件DAS 2.0非房室模型计算药动学参数并用统计软件SPSS 17.0对主要药动学参数进行比较。结果与正常大鼠体内药动学相比较,脑缺血-再灌注损伤对刺五加注射液中紫丁香苷产生蓄积,对异嗪皮啶的消除发生改变,对刺五加苷E无影响。结论初步推测脑缺血-再灌注损伤大鼠体内,紫丁香苷及异嗪皮啶与血浆蛋白结合能力发生改变,有待进一步研究。AIM To compare the pharmacokinetics of the normal and the cerebral ischemia-reperfusion rats given Acanthopanax Injection （ syringin, isofraxidin, method was for establishing the rat model with cerebral phy-tandem mass spectrometry （LC-MS/MS） was for and eleutheroside E） intravenously. METHODS Longa ischemia-reperfusion; high performance liquid chromatogra- determination of the contents of syringin, isofraxidin, and eleutheroside E in rat plasma. The pharmacokinetic parameters and statistics were obtained through DAS 2.0 and SPSS softwares processing, respectively. RESULTS While eleutheroside E remained unchanged between the two groups, gradual in vivo syringin accumulation and elimination change of isofraxidin were observed in cerebral ische- mia reperfusion rats, but not in normal rats. CONCLUSION We speculate from the study that the syringin and isofraxidin somehow combine with plasma proteins, but further study is needed.

关 键 词：脑缺血-再灌注损伤 大鼠 五加注射液 紫丁香苷 刺五加苷E 异嗪皮啶 药动学 

分 类 号：R969.1[医药卫生—药理学]