南赤瓟醇提物对裸鼠宫颈癌Caski移植瘤的抑制作用  

Effect and Mechanism of Alcohol Extraction from Thldiantha Nudiflora Hemsl on Growth of Human Cervical Cancer Caski Cells in Nude Mice

作　　者：王爱玲[1] 王海燕[2] 覃慧林 冯天艳 贺海波[1] 杨小姣[1] 邓为[1] 汪鋆植[1] 

机构地区：[1]三峡大学生物与制药学院&湖北省土家医药研究所,湖北宜昌443002 [2]湖北省宜昌市中医医院&三峡大学中医临床医学院,湖北宜昌443002 [3]神农架聚能药业有限公司,湖北神农架422400

出　　处：《三峡大学学报（自然科学版）》2015年第3期108-112,共5页Journal of China Three Gorges University：Natural Sciences

基　　金：湖北省自然科学基金科研项目(2013CFB224);宜昌市科学技术局项目(A13-302a-14)

摘　　要：探讨了南赤瓟醇提物对裸鼠宫颈癌Caski移植瘤的抑制作用,并在此基础上探寻其可能的机制.将0.3mL Caski(4×107个/mL)细胞悬液接种于裸鼠右侧后腿背部皮下,接种2次,间隔时间1周,建立裸鼠宫颈癌Caski移植瘤模型.将移植瘤裸鼠随机分为模型组、南赤瓟醇提物组(200和400mg/kg)组,每组5只,每天给药1次,连续给药4周,停药次日处死裸鼠,测量裸鼠体重、移植瘤体积、瘤重,计算抑瘤率;光镜下观察移植瘤组织形态学变化;Annexin V-FITC/PI染色测定细胞凋亡率,荧光定量PCR测定Bcl-2、Bax和Caspase-3、Caspase-9mRNA表达,并计算Bcl-2与Bax比值.结果表明南赤瓟醇提物可显著降低移植瘤体积和抑瘤率,上调Bax和Caspase-3、Caspase-9mRNA表达,下调Bcl-2mRNA表达和Bcl-2与Bax比值,与模型组比较具有显著性差异(P<0.05或P<0.01).南赤瓟醇提取物对人宫颈癌Caski细胞移植瘤生长具有明显抑制作用,其作用机制与调节Bcl-2、Bax和Caspase-3、Caspase-9基因表达有关.The inhibitory action and mechanism of alcohol extraction from Thldiantha nudiflora Hemsl on the growth of nude mice with Caski cells subcutaneously transplanted tumor is explored.Nude mice model with transplanted tumor of human cervical cancer Caski cells are established successfully by injecting 0.3mL cells（4×107）under the right hind leg subcutaneous,twice for a week.Fifteen nude mice were divided into 3groups randomly,model group,alcohol extraction from Thldiantha nudiflora Hemsl group（200,400mg/kg）.Each group was 5,once a day for 4weeks.Then all mice were sacrificed 24 hafter the final medication,and the transplanted tumor mass volume and weight were measured to calculate the tumor inhibitory rate;the antitumor effect of alcohol extraction from Thldiantha nudiflora Hemsl group was evaluated.Morphology changes of transplanted tumor tissues were observed under light microscope after HE staining.Annexin V/PI double-staining was performed to detect apoptosis of Caski cells by flow cytometry;the expressions of Bcl-2,Bax,Caspase-3and Caspase-9mRNA were detected by real-time PCR;and Bcl-2/Bax ratio was analyzed.Alcohol extraction fromThldiantha nudiflora Hemsl might significantly decrease the tumor volumes and tumor inhibitory rate,up-regulate the mRNA expression levels of Bax,Caspase-3and Caspase-9,down-regulate expression level of Bcl-2and Bcl-2/Bax ratio compared with the negative control group（P〈0.05 P〈0.01,respectively）.Alcohol extraction from Thldiantha nudiflora Hemsl exerted significant inhibitory effect on the growth of transplanted tumors of human cervical cancer Caski cells in nude mice.The mechanism may be associated with regulating the expressions of the apoptosis-related genes Bcl-2,Bax and Caspase-9,Caspase-3.

关 键 词：南赤瓟醇提物 CASKI细胞 裸鼠 移植瘤 凋亡 

分 类 号：R284[医药卫生—中药学]