解郁育胞方对慢性应激大鼠子宫内膜容受性的影响  

Effect of Jieyuyubao Prescription on Endometrial Receptivity for the Chronic Stress Rats

作　　者：耿会转[1] 贺漪[2] 周顺长[3] 韦文双 方鸿浩 黎烈荣[1] 

机构地区：[1]湖北中医药大学,武汉430061 [2]武汉市第一医院妇产科,武汉430022 [3]华中科技大学同济医学院实验动物中心,武汉430030

出　　处：《医药导报》2015年第6期741-745,共5页Herald of Medicine

摘　　要：目的研究解郁育胞方对慢性应激大鼠的子宫内膜胞饮突、HOXa-10 mRNA的影响,探讨其对慢性应激大鼠子宫内膜容受性的调节机制。方法 SPF级SD大鼠48只,按体质量分层随机分为3组:正常对照组、模型对照组、实验药物组。模型对照组、实验药物组大鼠建立慢性应激模型。实验药物组于刺激第1天开始,每日9:00给予解郁育胞方溶液(相当于生药1 g·m L-1)灌胃,给药容量为11.8mL·kg-1,每日1次,至经阴道涂片检查确认妊娠后第4天停药。正常对照组、模型对照组每日9:00灌服纯化水11.8mL·kg-1。3组大鼠分别于第21天16:00按照雌∶雄=2∶1合笼,确认妊娠后第4天处死大鼠,透射电镜制片评价子宫内膜胞饮突的成熟度,实时荧光定量聚合酶链反应(Real-Time PCR)检测HOXa-10 mRNA含量。结果胞饮突成熟度:正常对照组评分(2.7±0.3)分,模型对照组组评分(1.3±0.3)分,实验药物组评分(2.1±0.2)分,实验药物组与正常对照组比较,差异无统计学意义(P>0.05);与模型对照组比较差异有统计学意义(P<0.05)。HOXa-10 mRNA的表达:模型对照组HOXa-10 mRNA水平(0.658±0.031)较正常对照组(0.965±0.102)显著下降(P<0.05),实验药物组(0.866±0.125),与正常对照组比较,差异无统计学意义(P>0.05)。结论解郁育胞方可能通过促进子宫内膜上皮细胞HOXa-10 mRNA的表达增强,从而使胞饮突的数量和成熟度增加,进而改善子宫内膜的容受性。Objective To study the effect of Jieyuyubao prescription on endometrial pinopodes and HOXa-10 mRNA inchronic stress rats , and reveal the regulatory mechanisms of the endometrial receptivity. Methods Totally 48 SPF SD rats were randomly divided into three groups with weight stratified： normal control group （group A）, model control group （group B） and experimental drug group （group C）. Rat model of chronic stress was established in group B and group C. On the first day of stimulation, Jieyuyubao solution （1 g·mL-1 ） was intragastrically administered （11. 8 mL·kg-1 ） to the rats of group C at 9：00 am, once daily, and stopped giving at the fourth day of pregnancy （pd4） after being confirmed by vaginal smears. Purified water （11. 8 mL·kg-1 ） was intragastrically administered to the rats of group A and group B at 9：00 am. All the rats were mated at the sex ratio of female：male = 2：1 at 16：00 pm on the 21st day. The rats were sacrificed at the pd4. Maturity of endometrium pinopodes was assessed under transmission electron microscope. HOXa-10 mRNA content was evaluated by real-time fluorescence-PCR. Results The maturity of pinopodes was as follows： the maturity score of normal control group, model control group and experimental group was （2. 7 ± 0. 3）, （1. 3 ± 0. 3）, and （2. 1 ± 0. 2）, respectively, with significant difference between experimental drug group and model control group （P〈0. 05）. HOXa-10 mRNA expression was significantly decreased in model control group （0. 658±0. 031） as compared with normal control group （0. 965±0. 102） （P〈0. 05）. Conclusion Jieyuyubao prescription maybe increase the number and maturity of pinopodes through promoting HOXa-10 mRNA expression in endometrial epithelium cells, and then improve endometrial receptivity.

关 键 词：解郁育胞方 应激 慢性 子宫内膜 容受性 

分 类 号：R289.5[医药卫生—方剂学]