云南省玉溪市玉米致死性坏死病毒原的分子鉴定  

Molecular identification of the viruses causing maize lethal necrosis disease in Yuxi,Yunnan Province

作　　者：李帅[1] 朱敏[1] 夏子豪 王廿[1] 周涛[1] 董家红[2] 陈永对[2] 张仲凯[2] 范在丰[1] 

机构地区：[1]中国农业大学植物病理学系,北京100193 [2]云南省农业科学院生物技术与种质资源研究所,昆明650223

出　　处：《植物保护》2015年第3期110-114,共5页Plant Protection

基　　金：国家科技重大专项(2013ZX08003-001);云南省科技计划项目(2012CH007)

摘　　要：玉米致死性坏死病是由玉米褪绿斑驳病毒(Maize chlorotic mottle virus,MCMV)和一种或多种马铃薯Y病毒科病毒复合侵染引起的。2014年1月在对云南省玉溪市玉米病毒病害的调查中发现了一些表现严重花叶、矮化叶片甚至整个植株坏死症状的玉米。对采集样品进行RT-PCR检测,所有样品中都同时检测到了MCMV和甘蔗花叶病毒(Sugarcane mosaic virus,SCMV),在一个样品中同时检测到了MCMV、SCMV和南方水稻黑条矮缩病毒(Southern rice black-streaked dwarf virus)。Maize lethal necrosis disease is caused by synergistic interaction between Maize chlorotic mottle virus （MCMV） and one or several potyviruses. Severe mosaic, leaf necrosis （even plant lethal necrosis） or rough dwarf were found during a survey on virus diseases in maize carried out in Yuxi District, Yunnan Province in January 2014. RT-PCR, molecular cloning and sequence analysis were performed to identify the viruses present in the sampled plants. The results showed that all of the diseased samples tested were co-infected with MCMV and Sug- arcane mosaic virus （SCMV） and one of the plants was co-infected with MCMV, SCMV and Southern rice black- streaked dwarf virus （SRBSDV）.

关 键 词：玉米致死性坏死病 玉米 RT-PCR 

分 类 号：S432.41[农业科学—植物病理学]