支气管哮喘患者血清IL-33、IL-35水平及相关性分析  

The expression of serum interleukin-33, interleukin-35 in patients with bronchial asthma and their correlation

作　　者：陈杰[1] 彭健[1] 张卓然[1] 程国平[1] 

机构地区：[1]黄石市第二医院呼吸内科,435002

出　　处：《国际医药卫生导报》2015年第12期1644-1647,共4页International Medicine and Health Guidance News

基　　金：湖北省自然科学基金指导性项目（2013CFC057）;黄石市医药卫生科研项目（2013-34）

摘　　要：目的 研究支气管哮喘患者血清IL-33、IL-35的表达水平,分析IL-33、IL-35间相关性.方法 采用酶联免疫法（ELISA）检测81例非细菌性感染所致中-重度支气管哮喘急性发作期患者治疗前及80名正常对照者血清IL-35、IL-33水平,采用血细胞分析仪检测白细胞数（WBC）、中性粒细胞绝对值（N）、嗜酸性粒细胞绝对值（EOS）.细胞培养实验分为空白对照组、细胞刺激素组和IL-35刺激组,ELISA法检测其培养上清IL-33浓度.统计分析支气管哮喘患者急性发作期与正常对照组血清中IL-35、IL-33、WBC、N、EOS,并分析IL-35、IL-33间的相关性.结果 ①哮喘组血WBC、N及EOS计数均高于正常对照组[（7.82±2.93）×109/L vs.（6.38±2.14）×109/L;（5.63±1.47）×109/L vs.（4.57±1.22）×109/L;（0.43±0.27）×109/L vs.（0.22±0.15）×109/L;P＜0.001].②哮喘组血清IL-33水平较正常对照组明显升高[（217.26±52.31）ng/L vs.（105.43±31.64）ng/L],IL-35水平较对照组明显降低[（156.71±24.29）ng/L vs.（311.62±35.28）ng/L],差异有统计学意义（P＜0.001）;总体IL-35与IL-33水平呈负相关（r=-0.803,P＜0.05）.③哮喘组经过IL-35刺激IL-33水平与仅细胞刺激素相比更低[（15.08±1.92）ng/L vs.（57.33±15.62）ng/L],差异有统计学意义（P＜0.01）.结论 IL-35、IL-33均参与了支气管哮喘的发病,支气管哮喘发作期存在各细胞因子间调节失衡.Objective To investigate the expression of serum interleukin-33 （IL-33）,interleukin-35 （IL-35） in patients with bronchial asthma and their correlation.Methods The expression of serum IL-33,IL-35 of 81 patients with moderate or severe bronchial asthma in acute episode not caused by bacterial infection were detected with ELISA before treatment,along with 80 normal controls.The numbers of white blood cell （WBC）,absolute neutrophil count （N）,eosinophil absolute value （EOS） were detected by blood cell analyzer.In cell culture experiments,the plasma sample was divided into 3 groups：control group,cell stimulating hormone group,and IL-35 group.After cell culture experiments,the expression of plasma IL-33 in each group was measured by ELISA.Compared the expression of serum IL-35,IL-33,WBC,N,EOS in patients with bronchial asthma in acute episode and normal controls,analyzed the correlation between IL-35 and IL-33.Results ① The numbers of WBC,N,EOS of bronchial asthma group were higher than those of control group [（7.82±2.93）× 109/L vs.（6.38±2.14）× 109/L;（5.63± 1.47）× 109/L vs.（4.57± 1.22）× 109/L;（0.43±0.27）× 109/L vs.（0.22±0.15） × 109/L;P〈0.001].② The expression of serum IL-33 of bronchial asthma group was significantly higher than that of control group [（217.26±52.31）ng/L vs.（105.43±31.64）ng/L],the expression of serum IL-35 was significantly lower than that of control group [（156.71±24.29）ng/L vs.（311.62±35.28）ng/L],with statistically significant differences （P〈0.05）.③The expression of serum IL-33 of patients with bronchial asthma undergoing IL-35 stimulating was lower that of patients with bronchial asthma undergoing cell stimulating hormone [（15.08±1.92）ng/L vs.（57.33±15.62）ng/L],with statistically significant difference （P〈0.05）.Conclusion IL-35,IL-33 were involved in the pathogenesis of bronchial asthma.Cytokines regulating unbalance existed in acute episode of bronchial asthma.

关 键 词：支气管哮喘 白介素33 白介素35 INTERLEUKIN-33 Interleukin-35 

分 类 号：R593[医药卫生—内科学]