纳米硫化铜与明胶蛋白质的直接键合作用  

Direct Bonding of Nano-copper Sulfide with Gelatin

作　　者：刘岑[1,2] 黎幼群[1,2] 唐世华[1,2] 邓秋[1,2] 

机构地区：[1]广西民族大学化学化工学院,广西南宁530006 [2]广西民族大学广西林产化学与工程重点实验室,广西南宁530006

出　　处：《广西民族大学学报（自然科学版）》2015年第2期81-86,共6页Journal of Guangxi Minzu University ：Natural Science Edition

基　　金：国家自然科学基金(21067001)

摘　　要：在pH=12.0的明胶溶液中,采用一锅化学反应法制得水溶性明胶/CuS纳米生物复合物.利用透射电子显微镜(TEM)、X射线粉末衍射(XRD)对复合物进行表征,显示纳米CuS颗粒为球状且表面被明胶大分子包覆.紫外-可见吸收光谱(UV-Vis)和傅里叶红外光谱(FT-IR)表明CuS与明胶肽链的酰胺基键合;CuS与明胶的键合,对明胶内源荧光有着较强的猝灭作用,为静态猝灭过程.由修正的Scatchard公式模型求出复合物的表观形成常数,结合位点数以及反应的热力学参数(△rHm=37.88kJ·mol-1;△rSm=216.77J·K-1·mol-1;△rGm=-57.44/-58.98/-60.69kJ·mol-1),结果表明反应是自发进行(△rGm<0),且为熵驱动(△rSm>0).The gelatin/CuS bionanocomposite was synthesized by the direct bonding of nano copper sulfide and gelatin in gelatin solution at pH = 12.0. The samples were characterized by TEM and XRD which shows that the spherical CuS particles were enshrouded by gelatin. It was speculated that CuS and gelatin bonded in amide group of gelatin by means of UV-Vis and FT-IR; the binding of CuS and gelatin has a strong ability to quench the intrinsic fluorescence of gelatin, which is a static quenching procedure. The apparent formation constant, binding sites and thermodynamic parameters（ ΔrHm = 37.88 kJ ·mol^-1; ΔrSm=216.77 J · K^-1 · mol^-1 ;ΔrGm= -57.44/-58.98/-60.69kJ ·mol^-1 ）were calculated by the modified Scatchard equation, the results indicate that the reaction was spontaneous （ΔrGm〈 0）, and entropy driven （ ΔrSm 〉 0）.

关 键 词：纳米CuS 明胶 纳米生物复合物 热力学 

分 类 号：O62[理学—有机化学]