一株耐镍寡养单胞菌的分离及鉴定  

Isolation and Characterization of a Nickel-resistant Stenotrophomonas Strain

作　　者：王鹤[1,2] 巫晶晶[1,2] 张金丽[1,2] 王洁[1,2] 贾成光 张亚平[1,2] 

机构地区：[1]集美大学食品与生物工程学院,福建厦门361021 [2]福建省食品微生物与酶工程重点实验室,福建厦门361021

出　　处：《集美大学学报（自然科学版）》2015年第3期186-192,共7页Journal of Jimei University：Natural Science

基　　金：国家自然科学基金资助项目(51004053);福建省高校杰出青年科研人才计划项目(JA11146);集美大学优秀骨干教师第二层次项目(2011B003);中国科学院城市环境与健康重点实验室开放基金项目(KLUEH201302)

摘　　要：从福建某五金厂综合污水中筛选到一株对Ni2+耐受性400 mg/L的菌株,对其形态学、16S r DNA序列及生理生化特征进行分析,并利用较佳生长条件对所得菌株进行Ni2+吸附验证.结果表明:该菌为革兰氏阴性杆菌,在牛肉膏蛋白胨培养基上形成的菌落,表面较光滑,边缘整齐;经16S r DNA序列比对及生理生化特征的分析,将菌株JMUZJ-1鉴定为寡养单胞菌(Stenotrophomonas sp.);菌株JMUZJ-1生长的较好条件是p H 7.0,温度30℃,Na Cl质量分数0.5%,在该条件下,菌株对Ni2+表现出良好的吸附性能.A nickel resistant strain was obtained from a hardware factory in Fujian province by selection of spontaneous mutants in wastewater containing Ni^2＋. Morphological tests, molecular identifications of 16S rDNA and biochemical characterization to this strain have been performed. The efficiency of the strain in ab- sorbing Ni^2＋ under optimal growing conditions has been analyzed. The results showed that the nickel-resistant strain was Gram-negative. The strain produces yellow pigmented colonies on medium including beef extract and peptone, with smooth surface and neat edge. Sequence analysis of 16S rDNA and biochemical character- istics identified the strain JMUI - 1 as Stenotrophomonas sp. The optimal growth condition for the nickel resist- ant strain is at pH 7.0, 30 ℃, containing 0. 5% NaCl solution, which allows JMUI - 1 a good absorption performance of Ni^2＋.

关 键 词：微生物 筛选 重金属 耐受菌 镍 

分 类 号：Q939.97[生物学—微生物学]