螺旋粉虱生物测定方法研究  

The study of bioassay methods on Aleurodicus dispersus Russell

作　　者：刘晓亮[1] 韦典[1] 唐文伟[1] 唐良德[1,2] 曾东强[1] 

机构地区：[1]广西大学农药与环境毒理研究所,广西南宁530005 [2]中国热带农业科学院环境与植物保护研究所

出　　处：《植物检疫》2015年第3期48-52,共5页Plant Quarantine

基　　金：公益性行业(农业)科研专项(201103026-4);国家自然科学基金项目(31160375;31201573)

摘　　要：通过比较浸叶法、药膜法和Potter喷雾法3种生测方法对螺旋粉虱成虫进行生物测定,确定了喷雾法是螺旋粉虱适宜的生测方法。优化了Potter喷雾法的操作因子,当喷雾量为1~2 m L、喷雾压力为50~100 k Pa、沉降时间为20~40 s时,Potter喷雾法的测定结果更准确、可靠。确证了采用螺旋粉虱自然混合日龄成虫作为试虫的可靠性。该方法可作为螺旋粉虱药剂筛选及抗药性监测的生物测定方法。The toxicity of insecticides against Aleurodicus dispersus were determined by leaf dipping method, residual film and potter-spraying method, and the results indicated that potter-spraying method was the best bioassay method for ,4. dispersus. It was found that the potter-spraying method was more accurate and reliable for A. dispersus, with the spray volume of 1-2mL, the spray pressure of 50-100 kPa, and the settling time of 20-40 s. The other experimental results showed it was feasible that adults of naturally mixed ages could be instead of adults of the same age in A. dispersus bioassay. Potter-spraying method can be use as a bioassay method for screening insecticides against A. dispersus and resistance monitoring of A. dispersus.

关 键 词：螺旋粉虱 生测方法 浸叶法 药膜法 Potter喷雾法 寄主材料 

分 类 号：S433.89[农业科学—农业昆虫与害虫防治]