南宁地区成人及儿童ITP患者体内血小板自身抗体特异性分布的研究  

Study on the distribution of auto-antibodies against platelet in adults and children with primary immune thrombocytopenia in Nanning

作　　者：周燕 钟周琳 刘金莲 

机构地区：[1]南宁输血医学研究所/南宁中心血站,广西南宁530007

出　　处：《国际检验医学杂志》2015年第11期1500-1501,1504,共3页International Journal of Laboratory Medicine

基　　金：广西科学研究与技术开发计划项目(08160-05);南宁市科学研究与技术开发计划项目(20133141);广西自然科学基金项目(2012GXNSFBA053127);广西自然科学基金项目(2013GXNSFBA019206)

摘　　要：目的研究血小板膜糖蛋白特异性自身抗体在成人及儿童原发免疫性血小板减少症(ITP)患者中分布的异同。方法应用酶联免疫吸附试验(PAKAUTO试剂盒)检测ITP组(83例)及非ITP组(58例)患者血小板自身抗体,并分析成人组ITP(46例),儿童组ITP(37例)的抗GPⅡb/Ⅲa、抗GPⅠb/Ⅸ及抗GPⅠa/Ⅱa自身抗体特异性分布规律。结果 ITP组血小板自身抗体阳性率为66.27%,高于非ITP组的6.90%,差异有统计学意义差异有统计学意义(P<0.05)。成人组女性ITP患者占60.87%,儿童组女性ITP患儿占64.86%,差异无统计学意义(P>0.05),但两组女性发病率均高于男性,差异有统计学意义(P<0.05)。成人组ITP患者血小板自身抗体阳性率为63.04%,儿童组ITP患儿抗体阳性率为70.27%,差异无统计学意义(P>0.05);且成人组与儿童组ITP患者血小板自身抗体特异性分布差异无统计学意义(P>0.05),均以抗GPⅡb/Ⅲa和抗GPⅠb/Ⅸ抗体多见。结论血小板自身抗体检测对ITP诊断、鉴别诊断及治疗均有重要的参考价值,GPⅠa/Ⅱa抗体介导的ITP很值得深入研究。Objective Study on the distribution of auto‐antibodies against platelet in adults and children with primary immune thrombocytopenia（ITP） .Methods Platelet auto‐antibodies were detected in 83 ITP patients and 58 non‐ITP patients by enzyme linked immunosorbent assay （PAKAUTO kit） .Anti‐GP Ⅱ b/Ⅲ a ,GP Ⅰ b/Ⅸ and GP Ⅰ a/Ⅱ a auto‐antibodies were analyzed for 46 a‐dults and 37 children with ITP .Results The positive rate of autoantibody was 66 .27% in ITP patients ,which was much higher than that of non‐ITP patients ,the difference was significant（P 〈 0 .05） .As for ITP patients ,female ITP patients in adult group were 28 ,while in children group were 24 ,the difference was not significant（P 〉 0 .05） ,but the morbidity of ITP in females was higher than that of males in both of the two groups ,the difference was significant（P〈 0 .05） .There was no significant difference on the distribution of platelet auto‐antibodies in adult and children group（P〉 0 .05） ,the main of them was resistance against GP Ⅱ b/Ⅲ a and GP Ⅰ b/Ⅸ antibodies .Conclusion The detection of platelet auto‐antibodies could distinguish ITP and non‐ITP patients ,and GP Ⅰ a/Ⅱ a has an important reference value about therapy .

关 键 词：血小板减少 血小板膜糖蛋白 自身抗体 分布 

分 类 号：R446.6[医药卫生—诊断学]