大肠埃希菌和肺炎克雷伯菌质粒型AmpC酶基因型的检测分析  

The analysis of the genotyping of plasmid-mediated AmpCβ-lactamases produced by clinical strains of Escherichia coli and Klebsiella pneumoniae

作　　者：郑港森[1] 刘赞赞 张加勤[1] 黄朝阳[1] 马晓波[1] 李庆阁[2] 宋秀宇 

机构地区：[1]厦门大学附属第一医院检验科,福建厦门361003 [2]厦门大学生命科学院生物医学科学系,福建厦门361005 [3]厦门市中心血站,福建厦门361004

出　　处：《国际检验医学杂志》2015年第11期1505-1506,共2页International Journal of Laboratory Medicine

基　　金：福建省自然科学基金项目(2013D002);福建省卫生厅青年基金项目(2010-2-90)

摘　　要：目的：针对该院临床分离大肠埃希菌和肺炎克雷伯菌质粒型 AmpC 酶基因型进行研究分析。方法收集2011年7月至2012年8月对头孢西丁不敏感无重复临床分离大肠埃希菌和肺炎克雷伯菌共176株，采用聚合酶链反应（PCR）法和扩增全基因序列分析大肠埃希菌和肺炎克雷伯菌产 AmpC 酶基因型。结果 PCR 结果显示，AmpC 酶基因（ampC 基因）阳性率为18．2％主要以 DHA 型为主，阳性率为59．4％，CIT 型为37．5％，EBC 为3．1％；其中，大肠埃希菌 ampC 基因阳性率为11．4％，以CIT 型为主，阳性率为77．8％，DHA 型和 EBC 型阳性率均为11．1％；肺炎克雷伯菌 ampC 基因阳性率为23．7％，以 DHA 型为主，阳性率为78．3％，CIT 型为21．7％。基因序列结果显示，DHA 型有18株为 DHA‐1基因型和1株摩根摩根菌 ampC 基因型，一致率97．0％，CIT 型有10株为 CMY‐2基因型，1株 CMY‐42基因型和1株 CMY‐4基因型；EBC 型为阴沟肠杆菌 ampC 基因型，一致率为99．0％。将32株基因序列提交 GenBank ，均被接受，其登录号为 KJ127248～ KJ127279。结论该院临床分离大肠埃希菌 ampC 基因主要以 CMY‐2型为主，而肺炎克雷伯菌主要以 DHA‐1型为主。Objective To investigate the genotype and epidemiology of plasmid‐mediated AmpC β‐lactamases produced by the clinical strains of Escherichia coli and Klebsiella pneumoniae .Methods A total of 176 clinical nonrepetitive cefoxitin non‐sensitivity isolates of Escherichia coli and Klebsiella pneumoniae was collected from July 2011 to August 2012 .Polymerase chain reaction （PCR） for AmpC enzyme gene amplification and DNA sequencing were carried out for genotype of AmpC beta‐lactamases .Results The results of PCR showed that the positive rate of ampC of the 176 strains of Escherichia coli and Klebsiella pneumoniae AmpC was 18 .2% ,mainly DHA type ,counting for 59 .4% ,CIT counting for 37 .5% ,EBC counting for 3 .1% .The positive rate of ampC of Escherichia coli was 11 .4% ,mainly CIT type ,counting for 77 .8% ,the positive rates of DHA type and EBC type both were 11 .1% .The positive rate of ampC of Klebsiella pneumoniae were 23 .7% ,mainly DHA type ,counting for 78 .3% ,CIT type count‐ing for 21 .7% .The results of DNA sequencing showed that there were 18 strains DHA‐1 type and 1 strain ampC gene type of Morganella morganii in DHA type strains ,the concordance rate was 97 .0% ,10 CIT type strains was CMY‐2 type ,1 strain was CMY‐42 ,one strain was CMY‐4 type ,EBC type was ampC gene type of Enterobacter cloacae ,the concordance rate was 99 .0% .A total of 32 strains of gene sequencing were registered as KJ127248 - KJ127279 in GenBank .Conclusion The main genotypes of plasmid‐mediated ampC enzyme produced by Escherichia coli and Klebsiella pneumoniae were CMY‐2 and DHA‐1 respectively .

关 键 词：Β-内酰胺酶 质粒 大肠埃希菌 肺炎克雷伯菌 

分 类 号：R446.5[医药卫生—诊断学]