乙型肝炎病毒外膜大蛋白临床意义及其与病毒复制的相关性  

Clinical significance of hepatitis B virus large surface protein and its correlation with hepatitis B virus replication

作　　者：钟小强[1] 吴正林[1] 徐韵靖 何涛君[1] 朱新建[1] 

机构地区：[1]深圳市第四人民医院检验科,广东深圳518033

出　　处：《国际检验医学杂志》2015年第11期1559-1560,共2页International Journal of Laboratory Medicine

摘　　要：目的：探讨乙型肝炎病毒（HBV）外膜大蛋白（LHBs）与 HBV 复制的相关性。方法随机收集深圳市第四人民医院2013年8～11月乙型肝炎患者血清标本170例，采用酶联免疫吸附试验（ELISA）检测 LHBs ，电化学发光法检测 HBV 表面抗原（HBsAg）及 HBV e 抗原（HBeAg），实时荧光定量聚合酶链反应方法检测 HBV‐DNA 。按 HBeAg 结果分为 HBeAg 阳性组（58例）和 HBeAg 阴性组（112例）。比较 LHBs 和 HBV‐DNA 的阳性率，同时分析 LHBs 水平与 HBsAg 浓度及 HBV‐DNA 拷贝数的相关性。结果 HBeAg 阳性患者血清中，LHBs 阳性率与 HBV‐DNA 阳性率差异无统计学意义（χ2＝0．342，P ＞0．05）；HBeAg 阴性患者血清中，LHBs 阳性率与 HBV‐DNA 阳性率有统计学意义（χ2＝5．349，P＜0．05）。 LHBs 水平与 HBV‐DNA 拷贝数（r＝0．979，P＜0．05）、HBsAg 浓度（r＝0．923，P＜0．05）呈正相关。结论 LHBs 是从蛋白水平反映乙型肝炎患者体内病毒复制情况的可靠指标，尤其是对 HBeAg 阴性患者抗病毒治疗和预后判断有重要意义。Objective To explore the clinical significance of hepatitis B virus（HBV） large surface protein （LHBs） and its corre‐lation with HBV replication .Methods A total of 170 patients infected with HBV were randomly collected from the Forth People′s Hospital of Shenzhen from August to November 2013 ,LHBs was measured byusing enzyme‐linked immunosorbent assay（ELISA） , HBV surface antigen and HBV e antigen were measured byusing electrochemiluminescence immunoassay ,HBV‐DNA was quantita‐tively measured by real‐time polymerase chain reaction .The objects were divided into HBeAg positive group （58 cases）and HBeAg negative group（112 cases） .The positive rate of LHBs and HBV‐DNA were compared ,the correlation of LHB and HBsAg ,HBV‐DNA were analyzed .Results The positive rates of LHBs and HBV‐DNA had no significant difference （χ2 = 0 .342 ,P 〉 0 .05） in HBeAg‐positive patients .The positive rate of LHBs was higher than that of HBV‐DNA in HBeAg‐negative patients （χ2 = 5 .349 , P〈 0 .05） .Serum LHBs level was respectively correlated with the serum HBV‐DNA copies （r= 0 .979 ,P〈 0 .0） and HBsAg con‐tent （r= 0 .923 ,P〈 0 .05 .Conclusion Serum LHBs measurement might serve as a reliable biochemical criterion in the reflection of HBV replication at protein level ,and it is also valuable to monitor treatment effect and prognosis of the disease ,especially in HBeAg‐negative patients .

关 键 词：乙型肝炎 乙型肝炎病毒外膜大蛋白 乙型肝炎病毒-DNA 

分 类 号：R512.62[医药卫生—内科学] R446.6[医药卫生—临床医学]