Phoenix^(tm) 100检测葡萄球菌现状分析  

Analysis of metabolic status of staphylococcus infection by Phoenix^(tm) 100

作　　者：艾彪[1] 周莉[1] 艾明华[1] 马青[1] 朱丽莎[1] 徐灵[1] 

机构地区：[1]长江大学附属第一人民医院,湖北荆州434000

出　　处：《国际检验医学杂志》2015年第11期1582-1583,共2页International Journal of Laboratory Medicine

摘　　要：目的了解住院患者血液感染葡萄球菌在Phoenixtm100的生化表型、耐药基因、毒素基因情况。方法采用Phoenixtm100和聚合酶链反应检测葡萄球菌生化表型,耐药基因MecA,毒素基因sea、seb、sec、see。结果 Phoenixtm100的46种生化表型除表皮葡萄球菌C-CLST结果阳性外,其他表型结果与期待值一致,符合率95%~99%,同时检出耐甲氧西林金黄色葡萄球菌6株,携带MecA菌4株,产β-内酰胺酶菌16株,红霉素诱导克林霉素耐药(STAmls)4株,携带肠毒基因5株。结论 Phoenixtm100的46种生化表型测定,有助于了解葡萄球菌区域性生活代谢及流行病学情况,同时可以发现葡萄球菌生化表型特异的株型。检出耐药基因MecA与荧光聚合酶链反应方法结果一致,Phoenixtm100在微生物检测方面所具有的自动化程度高,检测结果快速准确等优点,是其他方法无法替代的。Objective To analyze biochemical phenotype ,resistance genes ,toxin genes of staphylococcus infected in hospitalized patients by Phoenixtm 100 .Methods Using Phoenixtm 100 machine and polymerase chain reaction method to detect biochemical phe‐notype ,resistance genes MecA ,toxin genes sea、seb、sec 、see of staphylococcus .Results In addition to staphylococcus epidermidis C‐CLST being obtained positive results ,other 45 biochemical phenotypes were in accordance with expectation values（coincidence rate 95% - 99% ） ,moreover ,6 strains of methicillin‐resistant Staphylococcus aureus strains ,4 strains of MecA strains ,16 strains of β‐lact strains ,4 strains of Erythromycin induced by clindamycin resistant bacteria（STAmls） strains ,5 strains of Bowel poison gene strains were detected .Conclusion 46 biochemical phenotypes measurement using Phoenixtm 100 machine contribute to understand‐ing metabolism and epidemiology of biochemical phenotype ,it could also found specific biochemical phenotype of staphylococcus . The results of resistance genes MecA are according with that by real‐time polymerase chain reaction .Phoenixtm 100 is fast and accu‐rate and has high degree of automation .

关 键 词：Phoenix^tm100 葡萄球菌 耐药基因 毒素基因 聚合酶链反应 

分 类 号：R446.5[医药卫生—诊断学]