人脐血干细胞对兔后肢动脉球囊损伤后内膜增殖和内皮功能的影响  

Effects of human umbilical cord blood stem cell transplantation on arterial intima proliferation and endothelial function in the rabbit hindlimb artery after balloon injury

作　　者：王富军[1] 杜亚萍[1] 丁海霞[1] 侯琳[1] 檀淼[1] 

机构地区：[1]河北医科大学第四医院内分泌科,石家庄050011

出　　处：《中华细胞与干细胞杂志（电子版）》2015年第2期19-23,共5页Chinese Journal of Cell and Stem Cell（Electronic Edition）

摘　　要：目的探讨人脐血干细胞(HUCBSC)移植对兔后肢动脉球囊损伤后动脉局部内膜增殖和内皮功能的影响。方法新西兰大白兔24只,随机分为正常对照组(A组,8只)、球囊损伤组(B组,8只)及球囊损伤加干细胞移植组(C组,8只)。三组均于全麻下分离兔右侧股动脉;B、C两组均应用3 mm×40 mm球囊扩张股动脉,每次3 min,共3次,并牵拉球囊,形成局部内膜损伤;C组球囊扩张后,以2.5×105的HUCBSC数移植到损伤血管局部,A、B组注入等量的磷酸盐缓冲液(PBS)。术前和术后28 d采用放射免疫法和酶联免疫吸附试验(ELISA)分别测定血浆内皮素-1(ET-1)及血清一氧化氮(NO)水平,并取股动脉采用苏木素-伊红染色(H-E)测定血管内膜面积及内膜面积与中膜面积比值。采用方差分析和配对t检验进行统计分析。结果术后28 d,B组和C组均可见损伤血管内弹力膜多处断裂,有不同程度的内膜增生,C组内膜面积[(0.231±0.033)mm2]及内膜面积与中膜面积比值(0.967±0.193)明显低于B组[(0.589±0.038)mm2,2.365±0.376;t=19.632,P<0.01,t=9.405,P<0.01],但明显高于A组(正常对照组)。术后28 d,A、B、C三组静脉血浆ET-1水平分别为(79.91±7.36)ng/L,(131.51±12.88)ng/L,(103.98±11.13)ng/L,B组明显高于A组(t=8.014,P<0.01),C组也明显高于A组(t=4.329,P<0.01),但明显低于B组(t=4.634,P<0.01)。静脉血清NO水平分别为(120.33±9.02)μmol/L,(48.01±6.20)μmol/L,(102.76±9.73)μmol/L,B组明显低于A组(t=18.914,P<0.01),C组也明显低于A组(t=3.901,P<0.01),但明显高于B组(t=14.083,P<0.01)。结论 HUCBSC血管局部移植可抑制兔后肢动脉球囊损伤后受损血管新生内膜的增殖,促进损伤血管内皮功能的恢复。Objective To explore the effects of human umbilical cord blood stem cell transplantation on arterial intima proliferation and endothelial function in rabbit hindlimb artery after balloon injury. Methods 24 New Zealand white rabbits were divided into the normal control group （Group A, 8 rabbits）, the balloon injury group （Group B, 8 rabbits） and the balloon injury combined with stem cells transplantation group （Group C, 8 rabbits）. The rabbit＇s femoral artery was mobilized under general anesthesia. The femoral arteries were expanded using 3 mm × 40 mm balloon 3 times, 3 minutes each time, in Groups B and C to cause intima injury. In Group C, 2.5 × 10^5 HUCBSCs were transplanted into injured artery. Rabbits in Groups A and B reveived phosphate buffer solution （PBS）. The plasma endothelin-1 （ET-1） and serum nitric oxide （NO） levels were measured by radioimmunoassay and enzyme-linked immunosorbent assay （ELISA） respectively at days 0 and 28. The vascular intimal area and the ratio of intima to media area of femoral artery were measured using hematoxylin eosin staining （H-E） assay. Analysis of variance and paired t test were used for statistical analysis. Results 28 days after intervention, arterial elastic membranes were ruptured at several points and intimas were hyperplastic in injured arteries of Groups B and group C. Intimal area [ （0.231±0.033） mm^2] and the ratio （0.967 ± 0.193） of intima to media area in Group C were lower than those in Group B [ （0.589 ± 0.038） mm^2, 2.365 ± 0.3761 t = 19.632, P 〈 0.01, t = 9.405, P 〈 0.01], but significantly higher than those in Group A. 28 days after intervention, the venous plasma ET-1 levels were （79.91 ± 7.36）ng/L, （131.51 ± 12.88）ng/L, and （103.98 ± 11.13）ng/L in Groups A, B, and C respectively. Rabbits in Group B had significantly higher ET-1 levels than those in Group A （t = 8.014, P 〈 0.01）. Rabbit in Group C had significantly higher ET-1 levels than those in Group A （t = 4.329, P 〈 0.01

关 键 词：脐血干细胞移植 血管内膜 内皮 

分 类 号：R457.7[医药卫生—治疗学]