总丹参多酚酸预处理对脊髓缺血再灌注损伤的保护作用  

Protective effect of pretreatment with salvianolic acids on spinal cord ischemia-reperfusion injury in rats

作　　者：李夏静 陈瑞琦[1] 陈旭征[1] 曹治云[1] 廖联明[1] 

机构地区：[1]福建中医药大学中西医结合研究院肿瘤研究所,福州350108

出　　处：《中华细胞与干细胞杂志（电子版）》2015年第2期33-37,共5页Chinese Journal of Cell and Stem Cell（Electronic Edition）

摘　　要：目的探讨总丹参多酚酸(TSI)对大鼠脊髓缺血再灌注损伤(SCII)的神经保护作用以及评估TSI预处理对氧化应激的影响和功能修复的作用。方法将18只雄性SD大鼠随机分为假手术组、模型对照组、TSI(20 mg/kg)处理组,每组6只。采用夹闭左肾下腹主动脉的方法建立SCII模型,分别于再灌注6,12,24及48 h对大鼠后肢进行运动功能评分。各组大鼠再灌注48 h后,取其脊髓组织,并进行组织病理学检测和生化指标分析。药物组与对照组间运动功能评分的均值比较用非参数秩和检验(KruskalWallis)进行分析,药物组与对照组间MDA,SOD,CAT,GSH-Px含量测定的均值比较采用单因素方差分析。结果与假手术组相比,模型组脊髓组织丙二醛(MDA)的含量明显升高,从2.95 mM/mg上升到5.90 mM/mg(P=0.023),谷胱甘肽过氧化物酶(GSH-PX)活力显著增强,GSH-PX酶活力从0.31 U/mg升高至0.78 U/mg(P=0.001),而超氧化物歧化酶(SOD)活力、过氧化氢酶(CAT)活力明显降低;SOD酶活力从16.03 U/mg降低至8.37 U/mg(P=0.001),CAT酶活力从1.24 U/mg降低至0.54 U/mg(P=0.002)。动物后肢神经功能评分明显下降,于6 h时从21分降低至15.5分(P=0.001)。预先给与动物注射TSI(20 mg/kg)后,可以降低MDA浓度,从5.90 mM/mg降为3.27 mM/mg(P=0.0355)。抑制GSH-PX活性,从0.78 U/mg降为0.36 U/mg(P=0.001)和提高SOD和CAT活性,SOD酶活力从8.37 U/mg升高至14.79 U/mg(P=0.001),CAT酶活力从0.54 U/mg升高至0.89 U/mg(P=0.043),提高动物后肢神经功能评分从15.5分升高至17分(P=0.001),TSI组在光学显微镜下脊髓病理改变轻微,而对照组脊髓损伤较重(P<0.05)。结论 TSI可通过改善氧化和抗氧化系统的平衡来预防SCII。Objectives The aim of this study was to investigate the neuroprotective effects of total salvianolic acids on spinal cord ischemia/reperfusion injury （SCII） in the rat and to assess the effects of salvianolic acids treatment on oxidative stress as well as functional recovery. Methods Eighteen adult male SD rats were randomly assigned to either sham, vehicle, or TSI （20 mg/kg） groups （n = 6 per group）. Spinal cord ischemia was induced by infrarenal aortic occlusion followed by 48 h of reperfusion. Hind limb motor function was assessed with Tarlov grades at 6, 12, 24 and 48 hours after reperfusion. Spinal cord tissues were harvested at 48 h after SCII. Histopathological and biochemical examinations were performed. The Kruskal-Wallis one-way analysis of variance by ranks was used for testing hind limb motor function. Differences for malondialdehyde （MDA）, gluthatione peroxidase （GSH-Px）, superoxide dismutase （SOD） and catalase （CAT） levels were tested using one-way analysis of variance. Results Compared with the sham-operated rats, the vehicle-treated SCII group showed significantly higher malondialdehyde （MDA） levels （2.95 mM/mg vs 5.90 mmM/ mg, P =0.023） and increased gluthatione peroxidase （GSH-Px） activity （0.31 U/mg vs 0.78 U/mg, P =0.001 ）. Superoxide dismutase （SOD） （16.03 U/mg vs 8.37 U/mg, P =0.001 ） and catalase （CAT） （1.24 U/mg vs 0.54 U/mg, P =0.002） activities were significantly reduced following SCII; Administration of TSI before and after SCII could reverse this trend. Furthermore, the percents of histological positive ceils were lower in the TSI-treated group compared with the vehicle-treated group （P 〈 0.05）. Conclusions These results suggest that TSI reduces spinal cord oxidative injury and facilitates repair following SCII by improving oxidant and anti-oxidant balance.

关 键 词：总丹参多酚酸 脊髓损伤 再灌注损伤 

分 类 号：R651.2[医药卫生—外科学]