南葶苈子化学拆分工艺研究  

Research on Fraction-splitting Technology of Seeds of Descurainia Sophia

作　　者：张明辉[1,2] 张艳丽[1,2] 赵威[1,2] 王小兰[1,2] 冯卫生[1,2] 匡海学[3] 郑晓珂[1,2] 

机构地区：[1]河南中医学院药学院,郑州450046 [2]呼吸疾病诊疗与新药研发河南省协同创新中心,郑州450046 [3]黑龙江中医药大学药学院,哈尔滨150040

出　　处：《世界科学技术-中医药现代化》2015年第3期476-484,共9页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家重点基础研究发展计划"973计划"项目(2013CB531802):宣泻利水中药的药性研究;负责人:郑晓珂

摘　　要：目的:建立南葶苈子的化学组分拆分方法,并对拆分组分进行稳定性和互不交叉性考察。方法:采用煎煮、蒸馏、萃取、醇沉、大孔吸附树脂等技术获得南葶苈子拆分组分,采用HPLC及化学计量学软件进行稳定性和互不交叉性评价。结果:将南葶苈子拆分为6个组分,HPLC色谱数据及化学计量学分析结果显示拆分工艺稳定,各拆分组分互不交叉,符合可拆分可组合的研究模式。结论:建立了南葶苈子化学组分拆分方法,各拆分组分互不交叉,工艺稳定性、重现性良好。This study was aimed to establish the method for fraction-splitting of seeds of Descurainia sophia, in order to study the stability and unoverlapping property of fractions of seeds of D. sophia. The fraction-splitting of seeds of D. sophia were obtained through the combination of various methods including decoction, distillation, extraction, ethanol precipitation and gradient elution of macroporous adsorption resin. HPLC and chemometrics software were used to analyze the stability and unoverlapping property of the fractions of D. sophia. The results showed that the chemical components from seeds of D. sophia was divided into six fractions. HPLC data and chemometrics analysis showed good stability of technology. The fraction-splitting of seeds of D. sophia was unoverlapping. It complied with the research model of chemical constituents of seeds of D. sophia which can be split and combinatorial. It was concluded that the established method for splitting fractions of seeds of D. sophia had good stability and repeatability. Each splitting fraction uncrossed others.

关 键 词：南葶苈子 化学组分拆分 互不交叉 化学计量学分析 

分 类 号：R284.1[医药卫生—中药学]