葶苈子醇沉组分免疫调节作用研究  

Experimental Study on Immunomodulatory Effect of Ethanol Sediments of the seeds of Descurainia sophia(L.) Webb. ex Prantl.

作　　者：郑晓珂[1,2] 杨梦[1,2] 白义萍 郭月婷[1,2] 张明辉[1,2] 匡海学[3] 冯卫生[1,2] 

机构地区：[1]河南中医学院药学院,郑州450046 [2]呼吸疾病诊疗与新药研发河南省协同创新中心,郑州450046 [3]黑龙江中医药大学药学院,哈尔滨150040

出　　处：《世界科学技术-中医药现代化》2015年第3期507-513,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家重点基础研究发展计划"973计划"项目(2013CB531802):宣泻利水中药的药性研究;负责人:郑晓珂

摘　　要：目的:研究葶苈子醇沉组分的体内外免疫调节作用。方法:采用体外淋巴细胞增殖实验考查葶苈子醇沉组分对正常小鼠脾脏中T淋巴细胞和B淋巴细胞增殖的影响;采用碳廓清实验、血清溶血素实验、迟发型超敏反应实验,考察葶苈子醇沉组分对环磷酰胺诱导的免疫低下小鼠非特异性免疫、体液免疫和细胞免疫的影响;考察葶苈子醇沉组分对免疫低下小鼠免疫器官和血清中细胞因子含量的影响。结果:葶苈子醇沉组分能够促进刀豆蛋白A(Con A)诱导的T淋巴细胞的增殖和脂多糖(LPS)诱导的B淋巴细胞的增殖(P<0.01);提高免疫低下小鼠碳廓清指数K、吞噬指数α、半数溶血值HC50和耳廓肿胀度(P<0.05或P<0.01);缓解免疫低下小鼠体质量的降低及胸腺和脾脏的萎缩(P<0.05或P<0.01),提高血清中IL-2、IFN-γ和IL-4的含量(P<0.05或P<0.01)。结论:葶苈子醇沉组分能够促进体外淋巴细胞的增殖,保护免疫低下小鼠的免疫器官,增强免疫低下小鼠的非特异性免疫和特异性免疫,具有免疫促进作用。This article was aimed to study the immunomodulatory effect of ethanol sediments of the seeds of Descurainia sophia （L.） Webb. ex Prantl. both in vitro and in vivo. The lymphocyte proliferation test in vitro was carried out to explore the effect of the ethanol sediments on the proliferation of T cell and B cell in the spleen of normal mice. And, the carbon clearance test, serum hemolysin test, and delayed-type hypersensitivity test were used to investigate the influence of fraction on non-specific immunity, humoral immunity and cellular immunity in the immunosuppressive mice induced by cyclophosphamide. Besides, the immunosuppressive model was used to evaluate the effect of fraction on immune organs and content of cellular factors in blood serum. The results showed that the ethanol sediments promoted Concanavalin A （Con A） induced T cell and Lipopolysaccharides （LPS） induced B cell （P 〈 0.01）. it increased the carbon clearance index K, phagocytic index a, half value hemolysis （HCS0）, and swelling degree of auricula （P 〈 0.05 or P 〈 0.01）. It reduced the body weight and atrophy of thymus and spleen index （P 〈 0.05 or P 〈 0.01）. It increased the contents of IL-2, IFN-7 and IL-4 in serum in immunosuppressive mice （P 〈 0.05 or P 〈 0.01）. It was concluded that ethanol sediments of the seeds of D. sophia （L.） Webb. ex Prantl. can boost the lymphocyte proliferation, protect the immune organs, and enhance the non-specific and specific immunity in immunosuppressive mice, which indicated that it had immune-promotion effect.

关 键 词：葶苈子 醇沉组分 免疫低下模型 免疫调节 

分 类 号：R285.5[医药卫生—中药学]