石榴叶鞣质和鞣花酸体外对大鼠原代脂肪细胞转化及脂代谢相关因子表达的影响  

Effects of Pomegranate Leaf Tennis and Ellagic Acid on In Vitro Primary Rat Adipocyte Transformation and Lipolytic-related Factor Expression

作　　者：余煊[1] 卢希[1] 姜敬非[1] 王欣佩[1] 邢东明[1] 雷帆[1] 杜力军[1] 

机构地区：[1]清华大学蛋白质科学教育部重点实验室生命科学学院医学院药物药理实验室,北京100084

出　　处：《世界科学技术-中医药现代化》2015年第3期520-525,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上项目(30801523):石榴叶总酚成分对脑缺血过程中PPARγ转录活性的影响及其脑保护作用研究;负责人:雷帆;清华大学实验室开放基金(LF20082377):调血脂药石榴叶鞣质质量标准关键技术研究;负责人:杜力军;科学技术部"重大新药创制"科技重大专项"十一五"计划课题(2011ZX09101-002-11):高低降脂胶囊治疗高脂血症Ⅲ期临床研究;负责人:邢东明;科学技术部"重大新药创制"科技重大专项"十二五"计划课题(2012ZX09103-201-041):糖脂代谢新药BLP的研究;负责人:邢东明

摘　　要：目的:探讨石榴叶鞣质及其鞣花酸对原代大鼠脂肪细胞分化及其相关脂代谢影响因子表达的影响。方法:利用体外培养原代大鼠前脂肪细胞方法,观察石榴叶鞣质及其鞣花酸对脂肪细胞内脂生成及其相关因子m RNA表达的影响。结果:石榴叶鞣质和鞣花酸对脂肪细胞中脂的形成具有明显的抑制作用,对脂蛋白脂酶(LPL)和葡萄糖-3-磷酸脱氢酶(GPDH)活性有一定的抑制作用,促进脂分解,降低胞内脂含量。并且上调PPARγ和脂肪酸结合蛋白(a P2),下调肥胖基因(ob)。结论:石榴叶鞣质可以抑制脂肪细胞内脂的生成,并能促进脂代谢,鞣花酸是其主要活性成分,具有与石榴叶鞣质相同的作用。This study was aimed to observe effect of pomegranate leaf tannin and ellagic acid on primary rat adipocyte differentiation and lipid metabolism-related factor expression. Primary rat preadipocyte was in vitro cultured to observe the effect of pomegranate leaf tannin and ellagic acid on lipid fat cells as well as mRNA expression of its related factor. The results showed that pomegranate leaf tannin and ellagic acid had obvious inhibition effect on fat formation in fat cells. It had certain inhibition effect on activities of lipoprotein lipase （LPL） and glucose- 3-phosphate dehydrogenase （GPDH）. It promoted fat decomposition and reduced intracellular lipid content. It upregulated PPAR7 and fatty acid-binding protein （aP2）. It downregulated obese （oh） gene level. It was concluded that pomegranate leaf tannin can inhibit fat generation of fat cells and promote fat metabolism. Ellagic acid was its main active ingredient, which had the same effect as pomegranate leaf tannin.

关 键 词：石榴叶 鞣花酸 脂肪细胞 脂代谢 

分 类 号：R285.5[医药卫生—中药学]