雪菊不同提取部位对3T3-L1前脂肪细胞增殖与分化的影响  

Effect of Coreopsis tinctoria Nutt. from Different Extract Regions on Proliferation and Differentiation of 3T3-L1 Preadipocytes

作　　者：贾明贤 张媛[1] 董世芬[1] 戴待[1] 余婕[1] 古文杰[1] 李志远[1] 王晶[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《世界科学技术-中医药现代化》2015年第3期544-549,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委青年基金项目(8120299):基于组分协同效应的维药雪菊防治冠心病的物质基础和作用机理研究;负责人:张媛;北京中医药大学自主选题项目(2014-JYBZZ-XS-070):雪菊不同提取部位对3T3-L1细胞协同作用的研究;负责人:贾明贤

摘　　要：目的:以3T3-L1前脂肪细胞为载体,考察雪菊总提物、正丁醇部位、黄酮单体CB-1及CB-2对该细胞增殖与分化的影响。方法:雪菊4个不同提取部位分别设3个剂量组。MTT法检测雪菊4个不同提取部位对3T3-L1细胞增殖的作用,油红O染色并通过比色分析细胞分化过程中胞浆脂质的形成与积累。结果:与对照组相比,雪菊总提取物100μg·m L-1,正丁醇部位0.5、5、50μg·m L-1,黄酮单体CB-1、CB-2在50μg·m L-1浓度时对细胞增殖有显著抑制作用(P<0.01);正丁醇部位的作用呈一定的量效关系(相关系数r=-0.903)。油红O染色结果表明,与对照组相比,雪菊总提取物1、10、100μg·m L-1均可以显著抑制细胞分化,减少细胞中脂质的形成(P<0.01);正丁醇部位能够剂量依赖性地抑制细胞分化(r=-0.779);CB-1与CB-2在50μg·m L-1浓度时对细胞分化有显著抑制作用(P<0.01)。结论:雪菊总提物、正丁醇部位、CB-1与CB-2均能够抑制3T3-L1前脂肪细胞的增殖与分化,减少细胞中脂质积累。The 3T3-L1 preadipocytes were used as carriers in the investigation of total extract, n-butanol extract, CB-1 and CB-2 of Coreopsis tinctoria Nutt. on cell proliferation and differentiation. Three groups at different doses were set for each of the four extract regions of C. tinctoria Nutt., respectively. MTT assay was used to detect 3T3- L1 cell proliferation by four extract regions of C. tinctoria Nutt. Oil Red O staining was used to analyze the formation and accumulation of cytoplasmic lipid during cell differentiation. The results showed that compared with the control group, there were significant inhibition on cell proliferation when the total extract of C. tinctoria Nutt. at 100 μg mL-1, n-butanol extract at 0.5, 5, and 50 μg mL-1, CB-1 and CB-2 at 50 μg mL-2 （P〈 0.01）. N-butanol extract showed certain dose-dependent manner （r = -0.903）. Oil Red O staining showed that compared with the control group, the total extract of C. tinctoria Nutt. at 1, 10, 100 μg mL-1 can obviously inhibit cell differentiation, reduce the formation of cytoplasmic lipid （P 〈 0.01）. N-butanol extract can inhibit cell differentiation in a dose-dependent manner （r = -0.779）. CB-1 and CB-2 obviously inhibited cell differentiation at the concentration of 50 μg mL-1 （P 〈 0.01）. It was concluded that the total extract, n-butanol extract, CB-1 and CB-2 of C. tinctoria Nutt. can inhibit the proliferation and differentiation of 3T3-L1 preadipocytes and reduce the formation of cytoplasmic lipid.

关 键 词：雪菊 3T3-L1前脂肪细胞 增殖 分化 

分 类 号：R285.5[医药卫生—中药学]