南方红豆杉总黄酮、总多糖与紫杉醇配伍增效减毒作用研究  

Study on Enhancing Efficacy and Reducing Toxicity of Total Flavonoids, Total Polysaccharides in Taxus mairei Compatibility with Taxol

作　　者：蔡伟[1] 夏苗芬[1] 熊耀康[2] 

机构地区：[1]浙江医药高等专科学校中药学院,宁波315100 [2]浙江中医药大学药学院,杭州310053

出　　处：《世界科学技术-中医药现代化》2015年第3期556-562,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：浙江省科技厅自然科学基金项目(LQ12H28003):基因与环境子对南方红豆杉质量形成机制的影响研究;负责人:张春椿;浙江医药高等专科学校校级科研项目(ZPCSR2012007):南方红豆杉紫杉烷类成分动态累积及其质量标准研究;负责人:蔡伟

摘　　要：目的:研究南方红豆杉中总黄酮、总多糖及其与紫杉醇配伍抗肿瘤的增效减毒作用。方法:采用MTT比色法,观察南方红豆杉中总黄酮、总多糖及其与紫杉醇配伍对4T1、A549、MCF-7细胞增殖抑制率的影响;利用等效线图探讨联合用药的行为。采用均匀设计分组,培养S180肉瘤ICR小鼠,观察配伍对荷瘤小鼠移植瘤生长的抑制情况;血液细胞分析仪检测血常规。结果:体外总多糖、总黄酮能够增强紫杉醇对4T1、A549细胞的抑制作用;总多糖能够增强紫杉醇体外对MCF-7细胞、体内对S180荷瘤小鼠移植瘤的抑制作用,体内减轻紫杉醇抗肿瘤时的骨髓抑制毒副作用。结论:紫杉醇可以通过配伍南方红豆杉总黄酮、总多糖达到增效减毒作用。This article was aimed to study enhancing efficacy and reducing toxicity on anti-tumor from total flavonoids and total polysaccharides in Taxus mairei compatibility with taxol. MTT assay was performed to analyze the cell proliferation and apoptosis of 4T1, A549, MCF-7 tumor cells after treatment of total flavonoids and total polysaccharides in T. mairei combined with taxol at different concentrations. Isobolograms was used to investigate the behavior of combination therapy. Therapy experiments were conducted on ICR mice bearing S180 sarcoma. The tumor weight was measured to calculate inhibition rate according to uniform design. Blood cells indicators were detected by blood cell analyzer. The results showed that total polysaecharides and total flavonoids can enhance the inhibition effect on 4T1, A549 cells by taxol. Total polysaccharides can enhance the inhibition effect of taxol to MCF-7 in vitro, and S180 sarcoma-bearing mice in vivo. It reduced the toxic effects of anti-tumor effect of taxol in vivo. It was concluded that taxol can achieve the effect of enhancing efficacy and reducing toxicity through combining with total flavonoids and total polysaccharides in T. mairei.

关 键 词：南方红豆杉 紫杉醇 总黄酮 总多糖 增效减毒 

分 类 号：R285.5[医药卫生—中药学]