HPLC法测定蒙药嘎日迪-13味丸中甘草苷、甘草酸铵、木香烃内酯、去氢木香内酯含量  

Determination of Liquiritin, Ammonium glycyrrhetate, Costunolide and Dehydrocostus lactone in Mongolia Medicine Ga Ri Di-13 Pill by HPLC

作　　者：韩志强[1] 巴图德力根[1] 

机构地区：[1]内蒙古民族大学附属医院蒙药临床药理研究所,通辽028007

出　　处：《世界科学技术-中医药现代化》2015年第3期692-697,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家科技支撑计划基金资助项目(2012BAI27B0204):蒙医治疗银屑病;原发性血小板减少性紫癜;脑梗塞恢复期等优势病的主药孟根乌森乌日勒;齐顺保利尔;嘎日迪乌日勒等3种院内蒙药制剂的工艺优化;建立质量标准;临床不良反应研究;负责人:巴图德力根

摘　　要：目的:HPLC法同时测定蒙药嘎日迪-13中甘草苷、甘草酸铵、木香烃内酯和去氢木香内酯含量。方法:甘草苷核甘草酸铵色谱条件:迪马色谱柱(250 mm×4.6 mm,5μm);流动相:乙腈(A)-0.4%磷酸(B)梯度洗脱,0-10 min(16%-18%A),10-30 min(18%A),30-40 min(18%-27%A),40-85 min(27%-45%A),85-86 min(45%-16%A);柱温:30℃;检测波长:237 nm;流速:1 m L·min-1。木香烃内酯和去氢木香内酯色谱条件:迪马色谱柱(250 mm×4.6 mm,5μm);流动相:乙腈-水(65:35),检测波长:225 nm,柱温30℃,流速1.0 m L·min-1,结果:甘草苷、甘草酸铵线性范围分别为0.10-1.2μg(r=0.999 9)、0.341-4.092μg(r=1.000 0);平均回收率分别为97.07%和100.13%,RSD分别为1.00%和1.84%(n=5)。木香烃内酯和去氢木香内酯线性范围分别为0.12-1.2μg(r=1.000 0),0.106-1.06μg(r=1.000 0);平均回收率分别为98.44%和98.90%,RSD分别为2.21%和3.38%。结论:该方法专属性强,重复性好,可用于蒙药嘎日迪-13中甘草苷、甘草酸铵、木香烃内酯和去氢木香内酯含量测定。In this article, a method to determine the content of liquiritin, ammonium glycyrrhetate, costunolide and dehydrocostus lactone in Mongolia medicine Ga Ri Di-13 pill was established. The chromatographic condition for liquiritin and ammonium glycyrrhetate was listed as the below： with Dimma column （250 mm × 4.6 mm, 5 μm） as stationary phase; with acetonitrile （A）-0.4%phosphoric acid （B） as mobile phase; gradient elution： 0-10 min （16%- 18% A）, 10-30 min （18% A）, 30-40 min （18%-27% A）, 40-85 min （27%-45% A）, and 85-86 min （45%-16% A）; column temperature was set at 30℃ ; detection wavelength was 237 nm; and flow velocity was 1 mL min-1. The chromatographic column condition for costunalide and dehydrocostus lactone was listed as the below： with Dimma column （250 mm× 4.6 mm, 5 μm） as stationary phase; with acetonitrile-water （65：35） as mobile phase; detection wavelength was 225 nm; column temperature was set at 30℃ and flow velocity was 1.0 mL min-1. The linearity ranges of liquiritin, ammonium glycyrrhetate, costunolide, and dehydrocostus laetone were 0.1-1.2 μg （r=0.999 9）, 0.341- 4.092 μg （r=l.000 0）, 0.12-1.2 gg （r=1.000 0）, and 0.106-1.06 （r=1.000 0）, respectively; the average recovery rates were respective glycyrrhet 97.07%, 100.13%, 98.44%, and 98.90%, respectively; the RSD were 1.00%, 1.84%, 2.21% and 3.38%, ly. This method is specificity and reproducible, and can be used to determination of Iiquiritin, ammonium ate, costunolide and dehydrocostus lactone in Mongolia medieine Ga Ri Di -13 pill.

关 键 词：蒙药嘎日迪-13味丸 HPLC 甘草苷 甘草酸铵 木香烃内酯 去氢木香内酯 

分 类 号：R284.1[医药卫生—中药学]