花生种子休眠解除过程中相关基因的表达分析  

Expression Analysis of Genes Involved in Peanut Seed Dormancy Release(Arachis hypogaea L.)

作　　者：陈静[1,2] 江玲[1] 王春明[1] 胡晓辉[2] 翟虎渠[3] 万建民[1,4] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室/江苏省植物基因工程技术研究中心,江苏南京210095 [2]山东省花生研究所,山东青岛266100 [3]中国农业科学院,北京100081 [4]中国农业科学院作物科学研究所,北京100081

出　　处：《作物学报》2015年第6期845-860,共16页Acta Agronomica Sinica

基　　金：山东省农业科学院青年英才培养计划项目;山东省良种工程(高产优质花生新品种培育)项目;青岛民生计划项目(13-1-3-77-nsh)和青岛民生计划项目(14-2-3-34-nsh)资助

摘　　要：种子休眠性是花生重要的农艺性状,外源乙烯利能诱导花生种子休眠的解除,为了阐明乙烯利作用下花生种子休眠解除的分子机制,设置吸胀的休眠种子为对照,100 mg L–1乙烯利处理吸胀休眠种子后不同时间的样品(AE1、AE2、AE3)进行转录组分析,比较了花生种子休眠解除过程中ABA、GA、ETH、auxin相关基因的表达。结果表明,15个与GA、40个与ABA、60个与ETH、56个与auxin相关的unigenes在花生种子休眠解除过程中表现显著差异表达。荧光定量PCR结果显示,ABA合成关键基因Ah NCED2和代谢关键基因Ah CYP707A1在种子休眠解除过程中均受外源乙烯利诱导,表达差异显著;在休眠和无休眠种子吸胀萌发过程中,Ah NCED2和Ah CYP707A1的表达趋势不同,Ah NCED2对于种子休眠的维持发挥积极作用,而Ah CYP707A1对于种子休眠解除发挥积极作用。Seed dormancy is one of important agronomic traits in peanut （Arachis hypogaea L.）. Seed dormancy can be released with exogenous ethephon. To understand the molecular mechanisms of switches from dormancy to germination in peanut seeds underlying the role of ethephon, we preformed transcriptome analyses among imbibed dormant seeds as control and dor-mancy-released seeds （AE1, AE2, AE3） treated by 100 mg L–1exogenous ethephon, and compared the expression of unigenes related to ABA, GA, ETH and auxin. The results showed that there were 15, 40, 60, and 56 unigenes associated with GA, ABA, ETH, and auxin respectively, which were significantly differentially expressed unigenes during the process from dormancy to germination. The results of Real-time RT-PCR showed that the expressions ofAhNCED2andAhCYP707A1 were induced dis-tinctly by exogenous ethephon in seed dormancy released process. In dormant and non-dormant seed imbibition and germination processes, there were different roles between expresses ofAhNCED2 andAhCYP707A1.AhNCED2played a positive role in maintaining seed dormancy, whileAhCYP707A1played a positive role for seed dormancy breaking.

关 键 词：花生 休眠性 AhNCED2 AhCYP707A1 

分 类 号：S565.2[农业科学—作物学]