乌拉尔图小麦WRKY转录因子的筛选与分析  

Identification and Analysis of WRKY Transcription Factors in Triticum urartu

作　　者：马建辉[1] 张黛静[1] 高小龙[1] 邵云[1] 姜丽娜[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《作物学报》2015年第6期900-909,共10页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31401452);国家"十二五"科技支撑计划项目(2013BAD07B14);河南师范大学博士启动基金项目(qd13032)资助

摘　　要：WRKY转录因子广泛参与植物抗逆胁迫反应,全面分析挖掘小麦A染色体组供体物种乌拉尔图小麦中的WRKY转录因子,对进一步挖掘分析六倍体小麦WRKY转录因子的分子功能具有重要意义。本研究通过生物信息学分析,在乌拉尔图小麦基因组数据中鉴定得到62条全长WRKY转录因子,其中14条能被准确定位在染色体上,并发现2对WRKY转录因子发生了复制;通过进化树分析,发现62条WRKY转录因子被分为8个小亚族,且小亚族内部的基因结构相对保守。通过荧光定量分析所选基因在非生物胁迫下的表达模式,筛选到2条在不同非生物胁迫下均上调表达的WRKY转录因子,为进一步分析其功能打下基础。WRKY transcription factors have been found to be involved in the processes responding to various abiotic and biotic stresses in plants. The knowledge on WRKY transcription factors inTriticum urartu （AA） will facilitate the function study of WRKY transcription factors in hexaploid wheat （Triticum aestivum, AABBDD）. In this study, 62 WRKY transcription factors with full length of coding sequence （CDS） were selected fromTriticum urartu genome through bioinformatic analyses, in which 14 could be located on specific chromosomes and two pairs had been duplicated. These WRKY transcription factors were divided into eight subgroups by phylogenetic analysis and the exon–intron structure in individual subgroups was relatively conserved. Two WRKY transcription factors were selected for function validation, and their expressions consistently increased under diverse abiotic stresses by qRT-PCR assay.

关 键 词：乌拉尔图小麦 WRKY转录因子 进化分析 非生物胁迫 

分 类 号：S512.1[农业科学—作物学]