不同强度跑台运动对高脂饮食大鼠脂肪组织PPARγ/脂联素/TNF-αmRNA的影响  

Effect of Treadmill Exercise of Different Intensity on the PPARγ/ Adiponectin / TNF-α mRNA in Adipose Tissue of Rats Fed with High Fat Diet

作　　者：夏书宇[1] 

机构地区：[1]郑州大学体育学院,河南郑州450044

出　　处：《成都体育学院学报》2015年第3期98-102,共5页Journal of Chengdu Sport University

基　　金：2014rh河南省重点科技攻关项目(142102310117)

摘　　要：目的:观察不同强度跑台运动训练对高脂饮食大鼠脂肪组织PPARγ、脂联素、TNF-αmRNA表达的影响,探讨不同强度跑台运动通过PPARγ/脂联素/TNF-α发挥防治高脂饮食危害的可能机制。方法:SD雄性大鼠40只随机分为高脂饮食对照组(C)、高脂饮食小强度运动组(SE)、高脂饮食中等强度运动组(ME)和高脂饮食大强度运动组(HE)。C组不运动,SE组、ME组和HE组大鼠进行10周,每周6次,每次60分钟的运动训练。10周末取材,比较四组大鼠体重、脂体比、血清TG、TC、HDL-C水平、脂肪组织PPARγ、脂联素、TNF-αmRNA表达以及它们的血清水平。结果:C组大鼠体重和脂体比显著高于SE、ME、HE三组进行不同强度跑台运动的大鼠(P<0.01),SE、ME两组大鼠体重和脂体比显著高于HE组大鼠(P<0.05);C组大鼠血清TG、TC水平均显著高于SE、ME、HE三组进行不同强度跑台运动的大鼠(P<0.01),SE组大鼠血清TG、TC水平均显著高于ME、HE两组(P<0.05),ME组大鼠血清TG水平显著高于HE组(P<0.05),ME组大鼠血清HDL-C水平显著高于其它三组(P<0.05);C组大鼠PPARγ、脂联素mRNA及其血清水平均显著低于SE、ME、HE三组进行不同强度跑台运动的大鼠(P<0.01),ME组大鼠PPARγ、脂联素mRNA及其血清水平显著高于SE、HE两组大鼠(P<0.01)。C组大鼠TNF-αmRNA及其血清水平均显著高于SE、ME、HE三组进行不同强度运动的大鼠(P<0.05),ME组大鼠TNF-αmRNA及其血清水平显著低于SE、HE两组大鼠(P<0.05)。结论:中等强度的跑台运动可显著提高高脂饮食大鼠脂肪组织PPARγ、脂联素mRNA的表达水平,降低脂肪组织TNF-αmRNA的表达,提示跑台运动可能通过PPARγ/脂联素/TNF-α发挥了防治高脂饮食大鼠脂肪积累的作用。Objective ： The purpose of this experiment is to investigate the effect of treadmill training of different inten- sity on the PPARγ/adiponectin/TNF -α mRNA in adipose tissue. Methods： Forty male SD rats are randomly assigned to four high fat diet groups. Rats in exercise groups are trained through treadmill exercise by SE, ME, HE. Rats in exercise groups undergo treadmill exercise for 10 weeks（60min/day,6days/week）. At the end of experiment, all rats are killed and their blood, epididymal fat as well as perirenal fat are obtained. Atheroscle- rotic was observed. The PPARγ/adiponectin/TNF - α mRNA in adipose tissue are determined. Serum is tested by enzyme - linked immunosorbentassay. Results ： The weight increase of rats in exercise groups is lower than that in group C, and the highest intensity has the most obvious effect（P 〈0.01. The serum TG,TC in group C are the highest in the four groups （P 〈0.01 ）. Compared with groups ME HE, the serum TG,TC in group SE are lower （P 〈 0.05 ）. The serum HDL - C in group ME is the highest in the four groups （P 〈 0.05 ）. The lev- el of PPARγ/adiponectin mRNA expression in adipose tissue and the serum adiponectin of group ME are the highest, and group C is the lowest. No significant difference is found between group SE and group HE. The level of TNF - α mRNA expression in adipose tissue and the serum TNF - α of group C are the highest. Group ME is the lowest with no significant difference found between group SE and group HE. Conclusion： Loaded treadmill exercise with proper intensity has good effect on PPARγ, adiponectin mRNA expression in adipose tissue, which lowers the TNF - α mRNA expression in adipose tissue of rats fed with high fat diet. This indicates that exercise playes an active role in preventing excessive accumulation of fat induced by high fat diet.

关 键 词：运动 脂肪组织 PPARΓ 脂联素 TNF-Α 

分 类 号：G804.7[文化科学—运动人体科学]