静置培养MDCK细胞的生长及代谢动力学研究  

Growth and Metabolism Kinetic Research of MDCK Cells in Stationary Culture

作　　者：暴艳敏[1] 王家敏[1] 马祺 令世鑫[1] 马卫国[1] 马忠仁[3] 乔自林[1] 

机构地区：[1]西北民族大学甘肃省动物细胞工程技术研究中心,甘肃兰州730030 [2]兰州百灵生物技术有限公司,甘肃兰州730010 [3]西北民族大学生物工程与技术国家民委重点实验室,甘肃兰州730030

出　　处：《黑龙江农业科学》2015年第6期71-74,共4页Heilongjiang Agricultural Sciences

基　　金：国家民委领军人才计划资助项目;国家自然科学基金资助项目(31160033);甘肃省科技计划资助项目(1308RJZA142);西北民族大学中央高校基本科研业务费专项资金资助项目(31920150029;31920130066)

摘　　要：为研究MDCK细胞的生长及代谢动力学特征,试验采用低密度静置培养,每天测定细胞生长密度和活力,并利用多参数生化分析仪测定培养液中Gluc、Lac、Gln和NH4+的含量,计算细胞指数生长期的比代谢速率。结果表明:MDCK细胞生长曲线呈"S"型,最大增殖密度为53.8×104 upf·mL-1,倍增时间为24.2h;对数生长期Gluc和Gln的比代谢(消耗速率为-2.20 mg·(106cells·d)-1和-3.85μmol·(106cells·d)-1,Lac和NH4+的比生成)速率为2.25mg·(106cells·d)-1和2.22μmol·(106cells·d)-1。In order to research growth and metabolism dynamics characteristics of MDCK cells,low cell density cultured method was used to determine cell density and viability every day. The content of Gluc, Lac,Gln and NH4^＋ in medium were determined by multi-parameter biochemical analyzer, and the specific metabolic rate were calculated. The results showed that the growth curve of MDCK cells was ＂S＂ type,the largest density was 53.8× 10^4 upf· mL^-1 and the doubling time was 24. 2 h. Specific consumption rate of Gluc and Gln were -2.20 mg. （10^6cells·d）^-1 and -3.85 μmol·（10^6cells·d）^-1 in logarithmic phase,and specific generation rate of Lac and NH4^＋ were 2.25 mg. （10^6cells·d）^-1 and 2.22 μmol·（10^6cells·d）^-1.

关 键 词：MDCK细胞 生长特征 代谢动力学 

分 类 号：Q813[生物学—生物工程]