防风总色原酮的超声提取工艺及抗氧化作用研究  

Study on Extraction of Chromone fromSaposhnikovia divaricataby Ultrasonic Wave and Anti-Oxidation

作　　者：王士杰[1] 罗超[1] 韩凤波[1] 赵岩[2] 张连学[2] 

机构地区：[1]吉林农业科技学院中药学院,吉林吉林132101 [2]吉林农业大学中药材学院,吉林长春130118

出　　处：《黑龙江农业科学》2015年第6期101-105,共5页Heilongjiang Agricultural Sciences

基　　金：吉林省教育厅资助项目(111022013033);吉林农业科技学院博士启动基金资助项目(119022012009);吉林农业科技学院青年基金资助项目(119012013019);长白山动植物资源利用与保护吉林省高校重点实验室资助项目(2013S015);吉林省科技厅基础处资助项目(20140101150JC)

摘　　要：为进一步研究防风提取物的抗氧化活性,采用超声提取法,利用L16(45)正交试验设计优选了防风中总色原酮最佳提取工艺条件,研究了总色原酮提取物对二苯代苦味酰基自由基DPPH·和超氧阴离子自由基(O2-)的清除能力及防风总色原酮的抗氧化能力。结果表明:防风总色原酮最佳工艺条件为料液比40∶1,90%乙醇溶液,50℃下超声提取30min,提取频率90Hz,平均提取率达4.05%。防风总色原酮提取物清除DPPH·的IC50值为117.32mg·mL-1,表明防风总色原酮具有较强的抗氧化活性。In order to further research the antioxidant activity of extraction from Saposhnikovia divaricata ,optimum extraction condition of chromone from Saposhnikovia divaricata with L16 （4^5） orthogonal test by ultrasonic wave and its scavenging ability to DPPH·and O^2-. were studied. The results showed that the optimum extraction condition was as follow：liquid-solid ratio was 40：1,90% ethyl alcohol,extraction temperature 50℃, extraction time was 30 min,extraction frequency was 90 Hz,and the average extraction rate was 4.05%. The extraction total chromone for DPPH. IC50 was 117.32 mg·mL^-1 ,which indicated total chromone from Saposhnikovia divaricata had strong anti-oxidation activity.

关 键 词：防风 色原酮 超声提取工艺 抗氧化 

分 类 号：TQ464[化学工程—制药化工]