基于SSR标记构建白蜡种质资源分子身份证  

Establishment of Molecular ID for Fraxinus Germplasms Based on SSR Markers

作　　者：胡春龙[1] 张晨[1] 刘翠兰[2,3] 李丽[2,3] 燕丽萍[2,3] 吴德军[2,3] 夏阳[2,3] 邢世岩[1] 王开芳[2] 

机构地区：[1]山东农业大学林学院,山东泰安271018 [2]山东省林业科学研究院,山东济南250014 [3]山东省林木遗传改良重点实验室,山东济南250014

出　　处：《山东农业科学》2015年第5期6-9,22,共5页Shandong Agricultural Sciences

基　　金：国家科技支撑计划项目"白蜡种质资源发掘与创新利用"(2013BAD01B06-6);山东省农业良种工程项目"农业生物资源创新利用研究";山东省科技发展计划项目(2014GNC111006)

摘　　要：以山东省林业科学研究院寿光试验站收集的46份白蜡为试验材料，对白蜡种质资源分子身份证的构建进行探究。利用SSR标记技术对白蜡试材进行区分，从150对引物中筛选出11对SSR引物对选定白蜡试材进行扩增，每个引物可以检测到3-5个基因型，平均每对引物扩增出2．52条带和2．09条多态性带，平均多态性位点百分率为82．93％。将11对引物及其检测出的等位基因依次赋值，构建了供试白蜡种质资源的分子身份证，能够达到区分各品种的目的。Using 46 Fraxinus materials from the Shouguang Experimental Station of Shandong Academy of Forestry, the establishment of molecular ID for Fraxinus germplasms was studied. These Fraxinus materials were distinguished by SSR markers. Eleven pairs of primers screened from 150 pairs were used to amplify the selected Fraxinus materials. The results showed that 3 - 5 genotypes could be detected by each primer. The average number of bands and polymorphic bands were 2.52 and 2.09 amplified by every pair of primers. The average polymorphic loci percentage was 82.93 %. After assignment of 11 pairs of primers and their alleles, the molecular IDs were established for the tested Fraxinus germplasms, which could well distinguish the varie- ties of Fraxinus.

关 键 词：白蜡 SSR 分子身份证 

分 类 号：S687.1[农业科学—观赏园艺] Q781[农业科学—园艺学]