Genomic variation in the hybrids of white crucian carp and red crucian carp: evidence from ribosomal DNA  

作　　者：WANG Jing XIAO Jun ZENG Ming XU Kang TAO Min ZHANG Chun DUAN Wei LIU WenBin LUO KaiKun LIU Yun LIU ShaoJun 

机构地区：[1]Key Laboratory of Protein Chemistry and Fish Developmental Biology of Ministry of Education of China, College of Life Sciences, Hunan Normal University

出　　处：《Science China(Life Sciences)》2015年第6期590-601,共12页中国科学（生命科学英文版）

基　　金：supported by Major International Cooperation Projects of the National Natural Science Foundation of China(31210103918);the National Natural Science Foundation of China(31201980,31430088 and 31272651);the National High Technology Research and Development Program of China(2011AA100403);the Natural Science Foundation of Hunan Province,China(14JJ3072);Science-Technology Foundation of Hunan Province,China(2014FJ3084);Research Foundation of Education Bureau of Hunan Province,China(13C523);the Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province(20134486);the Construct Program of the Key Discipline in Hunan Province,and Construct Program of the National Key Discipline

摘　　要：In this study, we conducted a cross of white crucian carp （♀）xred crucian carp （♂） （WR）, and characterized the morphology, reproduction and genetics of the progeny. Different from parents, WR with the gray color showed the hybrid morphological traits of both parents. WR possessed normal gonads producing mature eggs or sperm, and exhibited high fertilization rate （90.2%） and high hatchery rate （80.5%）, which contributed to produce and enlarge the population. WR with the same DNA content as parents was a diploid fish with 100 chromosomes （2n=100）. Amplified ITS of 45S rDNA, in WR the sequences consisting of 884 bp bases of the entire ITS-1 region, 5.8 S region, and entire ITS-2 region. The sequences showed high similarity between WR and its parents and leaned towards male inheritance. In WR, NTS of 5S rDNA consisted of three length types with total 654 bp bases. From sequence analysis of NTS, WR shared 94.2% and 95.1% similarities with their female and male parent, respectively. Sequence analysis of ITS and NTS revealed that there existed recombination and variation in the hybrid progeny, which was the genetic base for adaptation and speciation. In conclusion, we obtained WR from hybridization and it exhibited hybrid traits in morphology and variation in genetic composition showing essential difference with its parents. The obtainment of WR has important significance in fish genetic breeding.In this study, we conducted a cross of white crucian carp(♀)×red crucian carp(♂)(WR), and characterized the morphology, reproduction and genetics of the progeny. Different from parents, WR with the gray color showed the hybrid morphological traits of both parents. WR possessed normal gonads producing mature eggs or sperm, and exhibited high fertilization rate(90.2%) and high hatchery rate(80.5%), which contributed to produce and enlarge the population. WR with the same DNA content as parents was a diploid fish with 100 chromosomes(2n=100). Amplified ITS of 45 S r DNA, in WR the sequences consisting of 884 bp bases of the entire ITS-1 region, 5.8 S region, and entire ITS-2 region. The sequences showed high similarity between WR and its parents and leaned towards male inheritance. In WR, NTS of 5S r DNA consisted of three length types with total 654 bp bases. From sequence analysis of NTS, WR shared 94.2% and 95.1% similarities with their female and male parent, respectively. Sequence analysis of ITS and NTS revealed that there existed recombination and variation in the hybrid progeny, which was the genetic base for adaptation and speciation. In conclusion, we obtained WR from hybridization and it exhibited hybrid traits in morphology and variation in genetic composition showing essential difference with its parents. The obtainment of WR has important significance in fish genetic breeding.

关 键 词：white crucian carp red crucian carp HYBRIDIZATION genomic variation 

分 类 号：S917.4[农业科学—水产科学]