阳离子表面活性剂存在下孔雀石绿与脱氧核糖核酸的作用及分析应用  

Interaction of Malachite Green with Deoxyribonucleic Acid in the Presence of Cationic Surfactant CTMAB and Analytical Application

作　　者：陈宇春[1] 徐昆[2] 李原芳[1] 黄承志[1] 

机构地区：[1]西南师范大学环境化学研究所,重庆400715 [2]西南交通大学峨眉分校基础课部,四川峨眉614202

出　　处：《西南师范大学学报（自然科学版）》2002年第4期617-621,共5页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：国家自然科学基金 (2 9875 0 19); 重庆市科技攻关项目 (99-5 772 )

摘　　要：在酸性条件和阳离子表面活性剂CTMAB存在下 ,染料孔雀石绿与DNA发生作用 ,在 30 0 0nm ,337 6nm以及 4 5 0 0～ 5 0 0 0nm范围内产生强烈的共振散射峰 .根据 337 6nm处的共振散射信号 ,可以测定 0～ 2 0 μg/mL的小牛胸腺DNA和鱼精子DNA .检测限分别达 6 4 3ng/mL和 4 4 7ng/mL 。In acid medium, dye malachite green (MG) and cationic surfactant cetyl trimethyl ammonium bromide (CTMAB) can react with Deoxyribonucleic acids (DNAs), resulting enhanced resonance light scattering (RLS) signals at the wavelength of 300 0 nm, 337 6 nm, and the range between 450 0-500 0 nm. By using the enhanced RLS intensity at 337 6nm, 0 0-2 0 μg/mL ctDNA and fsDNA can be determined with the determination limits of 64 3 ng/mL and 44 7 ng/mL, respectively. The present method was successfully applied to the determination of nucleic acid in synthetic samples.

关 键 词：脱氧核糖核酸 孔雀石绿 阳离子表面活性剂 共振光散射 生物分析化学 含量测定 

分 类 号：Q523.03[生物学—生物化学]