实时荧光定量逆转录-聚合酶链反应(RT-PCR)法与细胞培养法检测流行性感冒病毒的比较  

Comparison of Detection of Influenza Viruses between real-time Quantitative Reverse Transcriotion-polymerase Chain Reaction(RT-PCR) and Cell Culture

作　　者：姜红涛[1] 姚秀林[1] 

机构地区：[1]抚顺市疾病预防控制中心,辽宁抚顺113006

出　　处：《中外医疗》2015年第8期192-193,共2页China & Foreign Medical Treatment

摘　　要：目的比较实时荧光定量逆转录-聚合酶链反应(RT-PCR)法与细胞培养法检测流行性感冒病毒的差异。方法采用实时荧光定量RT-PCR及经典的狗肾传代细胞病毒分离2种方法 ,同时对流感监测点送检的80份疑似流感标本检测流感病毒。结果细胞培养病毒分离的阳性数为26份,实时荧光定量RT-PCR的阳性数为36份,好=8.1,P<0.005。结论 通过该实验,验证了实时荧光定量RT-PCR的确快速敏感,适用于实验室快速诊断。Objective Comparison the difference detection of influenza viruses between real-time quantitative reverse transcrio- tion-polymerase chain reaction （RT-PCR） and cell culture. Methods Using real-time quantitative RT-PCR and classic MDCK cells virus isolation two ways, censorship influenza surveillance point 80 copies of suspected flu specimens of influenza virus si- multaneously. Results Consequence The positive of virus isolation cells is 26 copies. The positive of real-time quantitative RT- PCR is 36 copies, X2=8.1, P〈0.005. Conclusion Through this experiment, verifying the real-time quantitative RT-PCR is really fast sensitive and suitable for rapid diagnostic laboratory.

关 键 词：实时荧光定量逆转录-聚合酶链反应 细胞培养法 流感病毒 

分 类 号：R4[医药卫生—临床医学]