驴生长激素基因序列初步分析  

Preliminary Analysis of the Growth Hormone Gene in Donkey

作　　者：陈建兴[1] 孙玉江[2,3] 潘庆杰[3] 陈海伟[1] 赵冰[1] 李冰[1] 

机构地区：[1]赤峰学院生命科学学院,内蒙古赤峰024000 [2]东营市农业科学研究所,山东东营257091 [3]青岛农业大学动物科技学院,山东青岛266109

出　　处：《湖北农业科学》2015年第7期1751-1754,共4页Hubei Agricultural Sciences

基　　金：内蒙古自治区高等学校科学研究项目(NJZC13306)

摘　　要：通过对驴(Equus asinus)GH基因序列进行生物信息学分析,结果表明,驴GH基因共包含5个外显子和4个内含子。5个外显子连接形成651 nt的CDS区序列,整个CDS区翻译出216个氨基酸构成的多肽。驴GH基因编码的216个氨基酸中,大多数氨基酸为亲水性氨基酸。原始肽链的前20多个氨基酸序列出现一个典型的疏水峰,为该原始肽链的信号肽片段。驴的GH基因中的氨基酸以亮氨酸所占的比例最高(13.89%,共出现30次),其次为丙氨酸(10.19%,共出现22次),最低的为色氨酸(0.93%,共出现2次)。驴生长激素的编码存在明显的密码子使用偏好。The GH sequence of donkey （Equus asinus） was analysed with bioinformatics. The results showed that the GH sequence of donkey had 5 exous and 4 introns. CDS region of 651 nt was formed when the 5 cxons were connected together. Original peptide chain was composed of 216 amino acids, translated from the whole CDS region sequence. Most of 216 amino acids encoded were hydrophilic. A typical hydrophobic peak appeared in the leading 20 more amino acids sequence of the original peptide chain. It was considered as a signal peptide fragment after analysis. Among the amino acids of donkey GH, Leucine （13.89%,appearing 30 times had the highest proportion）, followed by alanine （10.19%, appearing 22 times）, and tryptophan（0.93%, appearing a total of 2 times）. The encoding of growth hormone in donkey had obvious codon usage bias.

关 键 词：驴(Equus asinus) 生长激素 生物信息分析 密码子使用偏好 

分 类 号：Q81[生物学—生物工程]