黄瓜疫霉菌生防菌的筛选及鉴定  

Screening and Identification of Bio-control Agents Against Phytophthora melonis

作　　者：孙莉[1] 易图永[1,2,3] 粟智平 王雅菲[1] 魏润洁 董俊[1] 

机构地区：[1]湖南农业大学植物保护学院,湖南长沙410128 [2]植物病虫害生物学与防控湖南省重点实验室,湖南长沙410128 [3]植物疾病控制与利用湖南省高校重点实验室,湖南长沙410128 [4]烟台出入境检验检疫局,山东烟台264000

出　　处：《中国蔬菜》2015年第6期23-27,共5页China Vegetables

基　　金：农业部公益行业计划项目(201003004);湖南省高校创新平台开放基金项目(13K064)

摘　　要：为了筛选对黄瓜疫霉菌有抑制作用的生防菌,采用土壤稀释法从湖南、北京等地采集的土壤样品中分离到269株放线菌和细菌,以黄瓜疫霉菌为靶标菌,通过平板对峙培养法,筛选出2株对黄瓜疫霉菌有明显拮抗作用的生防菌株。采用5倍稀释发酵液对黄瓜疫霉菌进行抑菌活性检测,放线菌X54和细菌P3的抑制率达到70%以上。通过培养形态特征观察、生理生化试验及16Sr DNA分子鉴定,放线菌X54为弗吉尼亚链霉菌(Streptomyces virginiae),细菌P3为地衣芽孢杆菌(Bacillus licheniformis)。In order to screen effective antagonistic agents to Phytophthora melonis, 269 strains were isolated from collected soil in Hunan and Beijing with gradient dilution method. 2 strains showed obvious antagonistic effects to Phytophthora melonis by using agar plate dual culture, and strain X54 and strain P3 were finally screened out. The diluted 5 times filtration showed their inhibition rates were over 70%. Through observation of culture morphological characteristics, physiological and biochemical experiment, and molecular identification of 16S rDNA, we found that actinomyeete X54 was Streptomyces virginiae and bacterial P3 was Bacillus licheniformis.

关 键 词：黄瓜疫霉菌 生物防治 弗吉尼亚链霉菌 地衣芽孢杆菌 

分 类 号：S436.421[农业科学—农业昆虫与害虫防治] S476.1[农业科学—植物保护]