电针对盘源性神经根损伤保护机制的实验研究  

Experimental Study on Protection Mechanism of Electro-acupuncture on Discogenic Nerve Root Injury

作　　者：徐贵锋[1] 高令军[1] 

机构地区：[1]上海中医药大学附属市中医医院骨伤科,上海200071

出　　处：《上海中医药大学学报》2015年第3期81-85,94,共6页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：上海市教委预算内科研项目(09JW39)

摘　　要：目的：观察电针对盘源性神经根损伤模型受损神经根超微结构的影响,探讨电针对盘源性神经根损伤的保护机制。方法：SD雄性大鼠12只,分为正常组、模型3 d组、模型12 d组、电针组。正常组无管压处理,模型组管压造模,电针组造模后电针干预。术后3 d、12 d对大鼠进行灌注,取压迫区L5神经根,采用电镜和光镜观察神经根形态学变化,并测量髓鞘厚度和轴突直径。结果：电针组与模型12 d组比较,轴突排列相对有序,髓鞘崩解较轻;运动根大直径（≥6μm）轴突的平均髓鞘厚度以及感觉根中等直径（4.0-5.9μm,2.0-3.9μm）轴突平均髓鞘厚度均比模型12 d相应轴突髓鞘厚（P〈0.05）。结论：盘源性神经根损伤是神经根脱髓鞘和髓鞘再生的过程,电针可以减少此过程中因机械压迫而导致的神经根脱髓鞘,减轻轴突分布的紊乱,保护神经根。Objective： To explore the influence of electro- acupuncture on the ultra microstructure of the discogenic injured lumbar nerve root, aim to discuss protection mechanism of electro- acupuncture. Methods： 12 Sprague- Dawley rats were divided randomly into 4 groups （ each with 3 rats） ： the normal group in which the L5 dorsal and ventral roots were exposed only, the experimental groups （3 days and 12 days） in which the L5 dorsal and ventral nerve roots were compressed using a silicone tube, the electro-acupuncture group in which rats were treated with electro-acupuncture. Those rats were perfused at 3 and 12 days after surgery. The L5 nerve root that was compressed by silicone tube was harvested, and observed with the optic microscope and electron microscope. The myelin thickness and the axon diameter were measured manually according to unbiased stereology method. Results： Compared with the experimental group （12days）, electro-acupuncture group has less axonal demyelination, and the average myelin thickness of large axon （ 〉 6 μm） in ventral root and medium axons （ 4-5.9 μm, 2 - 3.9μm） in dorsal root in electro-acupuncture group was thicker. Conclusion： Chronic spinal nerve root compression could produce a recoverable damage to nerve root ; Electro- acupuncture may reduce axonal demyelination and improve the axonal distribution caused by the nerve root compression.

关 键 词：电针 神经根损伤 脱髓鞘 超微结构 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]