碧桃花瓣转录组微卫星特征分析  

Deep sequenced-based transcriptome analysis of microsatellites in peach(Prunus persica cv. duplex) flowers

作　　者：马秋月[1] 廖卓毅 张得芳[1] 戴晓港[1] 陈赢男[1] 李淑娴[1] 

机构地区：[1]南京林业大学南方现代林业协同创新中心,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2015年第3期34-38,共5页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家林业公益性行业科研专项重大项目(201304102);国家自然科学基金项目(31270711);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：为开发碧桃转录组微卫星信息,利用454高通量测序技术,对其花瓣转录组序列进行SSR位点发掘,结果发现含SSR的序列4 705条,共得到5 668个SSR,平均每3.49 kb出现1个SSR。微卫星序列主要以三碱基重复为主,约占总数的42.66%。笔者共发现516种碱基重复基元,所占比例最高的为(AG/CT)n(18.34%),其次是(AAG/CTT)n(12.42%)。微卫星多为重复长度小于20 bp的短序列,长度大于20 bp的微卫星仅占总数的12.13%。研究还发现碧桃花瓣微卫星的频率和长度呈显著负相关(P<0.05),相关系数为-0.246。To obtain information of Prunus persica cv. duplex, the transcriptome of peach flowers were sequenced by 454 high-throughput sequencing technology. A total of 5 668 SSRs were identified in 4 705 sequences, with an average densi-ty of one SSR per 3.49 kb. Tri-nucleotide repeats were the most abundant, accounted for 42.66% of all SSRs. Among all the 516 SSR motifs, （ AG/CT ） n was the most frequent repeat motif （ 18.34%） , followed by the （ AAG/CTT ） n （ 12.42%） . The microsatellites in length below 20 bp were in maximum proportion, while the microsatellites over 20 bp were only 12.13%. There were significant negative correlation （ P〈0.05） between the frequency of microsatellites and the length, the correlation coefficient was -0.246.

关 键 词：碧桃 微卫星 SSR 转录组 高通量测序 

分 类 号：Q948[生物学—植物学] Q78