年青人骨髓间充质干细胞来源生物活性因子对年老人骨髓间充质干细胞功能的影响  

Rejuvenation of old mesenchymal stem cells by young cell derived bioactive factors

作　　者：黎佼[1] 胡明[1] 李国强[1] 莫沛[1] 刘建卫[1] 曾波航[1] 董珺[1] 刘世明[1] 

机构地区：[1]广州医科大学附属第二医院广州心血管疾病研究所,广州510260

出　　处：《广东医学》2015年第10期1501-1504,共4页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:81401156);广州市医药卫生科技项目(编号:20141A011085)

摘　　要：目的探讨年青人骨髓间充质干细胞(h MSCs)来源生物活性因子对年老人h MSCs功能的影响。方法低氧无血清培养条件下获得年青及年老h MSCs条件培养基(CM)。将年老h MSCs置于年青h MSCs来源CM中,或相反将年青h MSCs置于年老h MSCs来源CM中。通过Transwell实验、油红O染色、茜素红染色、β半乳糖苷酶染色、qRT-PCR分别检测不同来源CM中生物活性因子对h MSCs迁移能力、向脂肪细胞及成骨细胞分化能力、衰老、microRNA表达的影响。结果年青h MSCs来源CM生物活性因子可促进年老h MSCs功能再生,增强迁移能力和向脂肪细胞及成骨细胞分化能力,减少衰老。相反,将年青h MSCs置于年老h MSCs来源CM中,年青h MSCs功能受损,迁移能力下降,向脂肪细胞及成骨细胞分化能力下降,衰老增加。qRT-PCR检测发现,年青h MSCs来源CM生物活性因子可促进h MSCs中miR-10a表达,而年老h MSCs来源CM生物活性因子可抑制其表达。结论年青h MSCs来源生物活性因子可促进miR-10a表达,促进h MSCs迁移、分化,减少细胞衰老,促进年老h MSCs功能再生。Objective To investigate the effects of human mesenchymal stem cells（ h MSCs） derived bioactive factors on h MSCs function.Methods Old h MSCs were exposed to a young cell derived condition medium（ CM）.Conversely,young h MSCs were exposed to an old cell derived CM.The transwell tests were used to detect the migration capability of h MSCs.The differentiation capability was compared by inducing h MSCs into adipogenic and osteogenic cells.The effect of condition medium bioactive factors on h MSCsenescence were detected by β-galactosidase staining.The changes in microRNA（ miRNA） expression were determined by quantitative real-time PCR（ qRT-PCR）.Results Old h MSCs can be rejuvenated by exposure to a young cell derived CM.The h MSCmigration and differentiation ability was increased,and senescence was decreased.Conversely,the migration and differentiation ability of young h MSCs was impaired by exposure to an old cell derived CM.And the senescence was increased.The expression of miR-10 a was down-regulated in the old h MSCs derived CM,but was up-regulated in the young h MSCs derived CM.Conclusion Young cell derived bioactive factors can rejuvenate old h MSC function by increasing the expression of miR-10 a.

关 键 词：人骨髓间充质干细胞 年龄 细胞迁移 分化 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]