满山红总黄酮对肠平滑肌收缩功能影响  

Effect of the Rhododendron Dauricum L. Total Flavonoids on the Contraction of Small Intestine Smooth Muscle and the Mechanism

作　　者：张博男[1,2] 张静怡[3] 储金秀[1,2] 韩婷[1,2] 韩淑英[1,2] 

机构地区：[1]河北联合大学基础医学院药理学系,河北唐山063000 [2]河北联合大学基础医学院唐山市慢性病临床基础研究重点实验室,河北唐山063000 [3]唐山市工人医院心内科,河北唐山063000

出　　处：《辽宁中医药大学学报》2015年第6期33-35,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：河北省自然科学基金项目(C2010001795);唐山市科技计划项目(14130224a);河北联合大学科学研究基金项目(Z201325)

摘　　要：目的：观察满山红总黄酮（Rhododendron dauricum L.total flavonoids,RTF）对肠平滑肌收缩功能的影响并探讨其作用机制。方法：采用炭末推进法,观察RTF对正常小鼠小肠蠕动的影响;采用离体小肠灌流技术,观察RTF对乙酰胆碱（Ach）、组胺和氯化钡所致肠平滑肌收缩的影响。结果：RTF（180、90、45 mg·kg^-1）可以剂量依赖性的降低炭末推进率（P〈0.01）;RTF（0.5-4 g·L^-1）对乙酰胆碱（Ach）、组胺和氯化钡所致肠平滑肌收缩也具有剂量依赖性的抑制作用（P〈0.01）。在无钙灌流液中,RTF（2 g·L^-1）孵育10 min后可使CaCl2的量效曲线向下移位且可使Ach在无钙灌流液中诱导的肠管最大收缩幅度明显降低（P〈0.01）。结论：RTF在体内外能剂量依赖性的抑制小肠平滑肌的收缩,其机制与阻断M3受体和H1受体、降低细胞内游离钙离子浓度有关。Objective ： To observe effect of the Rhododendron dauricum L. total flavonoids （ RTF ） on the contraction of small intestine smooth muscle and explore the mechanism. Method： Carbo medicinalis facilitation method was used to observe effect of RTF on creeping of normal mice small intestine ; the isothermal perfusion of small intestine in vitro was used to observe effect of RTF on the contraction induced by Ach, histamine and BaC12. Result： RTF （ 180,90,45 mg· kg^-1 ）decreased rate of earbo medicinalis facilitation with a dose-dependent manner （ P〈0.01 ）; RTF （ 0.5-4 g ·L^-1 ） also inhibited the contraction of small intestine smooth muscle induced by Ach, histamine and BaCl2 with a dose-dependent manner （ P〈0.01 ）. In Ca^2＋ free K-H solution pre-depolarized with KC1, both Ca^2＋ and Ach -induced contraction were dramatically attenuated after pretreated with RTF （ 2 g ·L^-1 ）for 10 rain in krebs solution without Ca^2＋ （ P〈0.01 ）. Conclusion ： RTF could inhibit the contraction of small intestine smooth muscle with a dosedependent manner in vivo and in vitro, and the mechanism may be related to antagonize the M3 and H1 receptor and reduce of mtracellular Ca concentration.

关 键 词：满山红 小肠平滑肌 钙离子 

分 类 号：R685.6[医药卫生—骨科学]