野菊花提取物中蛇床子素在大鼠血浆中药代动力学研究  

Study on the Pharmacokinetics of Osthole after Oral Administration of Chrysanthemum Indicum L. Extract in Rats

作　　者：吴学军[1] 邹荣华[1] 冷爱晶[2] 

机构地区：[1]沈阳东新药业有限公司,辽宁沈阳110330 [2]大连医科大学附属第一医院,辽宁大连110161

出　　处：《辽宁中医药大学学报》2015年第6期39-42,共4页Journal of Liaoning University of Traditional Chinese Medicine

摘　　要：目的：建立HPLC法测定3个剂量口服给药后,野菊花提取物大鼠血浆浓度的分析方法研究蛇床子素在大鼠体内的药物动力学特征。方法：选用Phenomsil C18色谱柱（5μm,250 mm×4.6 mm,斐纳米技术公司,北京）,以甲醇-0.4%乙酸（55∶45）作为流动相,流速为1 mL/min,检测波长为322 nm,以黄芩苷为内标物,采用HPLC法进行测定。结果：实验结果表明蛇床子素在2-400μg/mL范围内与色谱峰面积呈良好的线性关系,R≥0.990 2。该方法日内精密度、日间精密度及提取回收率均符合要求。结论：3种剂量12.5、25、50 mg.kg-1灌胃给药后,蛇床子素呈线性动力学过程,数据拟合选择权重系数1/C2,均符合二室开放药动学模型。该研究首次建立了一种测定口服给药后大鼠血浆中野菊花提取物蛇床子素的高效液相色谱法,操作简便,结果准确可靠。Objective：To investigate the pharmacokinetics of osthole of Chrysanthemum indieum L. extract in rat following oral administration. Methods ： The biological samples of the plasma after oral dosing were analyzed by a sensitive and specific HPLC method with baicalin as internal standard and methnol-0.4% acetic acid aqueous （ 55 ： 45 ） as mobile phase. Results ： The validated HPLC method was successively used for the quantitative analysis of the osthole of Chrysanthemum indicum L. in rat＇s plasma. Conclusion ： The results showed that osthole presented dose-dependent pharmacokinetics in the range of 12.5-50 mg · kg^-1 and osthole fitted a two-compartment open model. The method developed was suitable for the pharmacokinetic study of osthole in rats after oral administration of Chrysanthemum indicum L. extract in rat.

关 键 词：野菊花提取物 高效液相色谱法 药代动力学 大鼠 

分 类 号：R284.2[医药卫生—中药学]