福建汉族乙型肝炎肝硬化湿热证易感性与HLA-DRB1基因多态性的相关研究  

Relevant Research Between the Hepatitis-B Cirrhosis of the Liver of Damp Heat Syndrome of the Han in Fujian and the Human Leukocyte Antigen-DRB1 Gene Polymorphism

作　　者：吴同玉[1,2] 石志平[3] 李东良[4] 刘瑶[1] 张凌媛[1] 丁珊珊[1] 

机构地区：[1]福建中医药大学,福建福州350122 [2]福建省中医健康状态辨识重点实验室,福建福州350122 [3]武汉科技大学医院,湖北武汉430065 [4]南京军区福州总医院,福建福州350025

出　　处：《辽宁中医药大学学报》2015年第6期56-59,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金青年基金项目(81102559)

摘　　要：目的:研究HLA-DRB1基因多态性与福建汉族乙型肝炎肝硬化患者湿热证的易感性。方法:运用中医证素辨证方法研究乙型肝炎肝硬化患者的中医湿热证候特征,采用PCR-RFLP结合DNA测序仪的方法研究HLA-DRB1的基因型及等位基因频率。结果:福建地区汉族健康正常人HLA-DRB1基因出现12个位点,乙型肝炎肝硬化湿热证组和非湿热证组HLA-DRB1基因出现11个位点,通过χ2检验,结果显示正常组与湿热证组、非湿热证组三组比较中,HLA-DRB1*1502与DRB1*0301、DRB1*0405、DRB1*0406、DRB1*0407、DRB1*0408、DRB1*0801、DRB1*1101、DRB1*1104、DRB1*1110、DRB1*1501、DRB1*1601相比,差异均有统计学意义(P<0.05),与DRB1*1202、DRB1*0305相比,P=0.05;在乙型肝炎肝硬化湿热组和非湿热组中,HLA-DRB1*1502与DRB1*0301、DRB1*0405、DRB1*1101相比较,差异有统计学意义(P<0.05);DRB1*0301、DRB1*0405、DRB1*1101、DRB1*0406、DRB1*0407、DRB1*0408、DRB1*1104、DRB1*1110、DRB1*1501、DRB1*1601之间相比较,差异无统计学意义(P>0.05)。结论:福建地区汉族乙型肝炎肝硬化患者可能与HLA-DRB1*1502有关,尤其可能是湿热证患者的易患基因型,而DRB1*0301、DRB1*0405、DRB1*1101则不是湿热证的易患基因型。Objective ： To study the susceptibility between the hepatitis B cirrhosis of the liver of damp heat syndrome of the Han people in Fujian and the human leukocyte antigen-DRB1 Gene polymorphism. Methods ： Studying on the characteristics of TCM damp heat syndrome in patients with hepatitis B cirrhosis by the syndrome element methods, Using PCR-RFLP combined with DNA sequencing method on the study of HLA-DRB1 genotype and allele frequency. Results： There is 12 gene loci of Human leukocyte antigen- DRB1 gene in Han nationality of healthy people in Fujian area, There is 11 gene loci of Human leukocyte antigen-DRB 1 gene in hepatitis B liver cirrhosis of damp heat syndrome group and non damp heat syndrome group. Through the chi square test, the results showed that comparison of three groups in normal group and the damp heat syndrome group, non damp heat syndrome group, compared with HLA-DRB1＊1502 and DRB1＊0301, DRB1＊0405, DRB1＊0406, DRB1＊0407, DRB1＊0408, DRB1＊0801, DRB1＊1101, DHB1＊1104, DRB1＊1110, DRB1＊1501, DRB1＊1601, the differences were statistically significant （ P〈0.05 ）, compared with DRB 1＊ 1202, DRB 1＊0305, P=0.05, the results showed that comparison between the damp heat syndrome group and non damp heat syndrome group. HLA-DRB1＊1502 and DRB1＊0301, DRB1＊0405, DRB1＊1101, the difference was statistically significant （ P〈0.05 ）, DRB1＊0301 , DRB1＊0405, DRB1＊1101, DRB1＊0406, DRB1＊0407, DRB1＊0408, DRB1＊1104, DRB1＊1110, DRB 1 ＊ 1501, DRB 1 ＊ 1601, the difference was not statistically significant （ P〉0.05 ） .Conclusion ： It may be associated with HLA-DRB 1＊ 1502 in Han nationality hepatitis B cirrhosis patients in Fujian area, especially susceptible to the people of damp heat syndrome, The genotype of DRB 1 ＊0301, DRB 1＊0405, DRB 1 ＊ 1101 is not susceptible to the people of damp heat syndrome.

关 键 词：湿热证 证候易感性 人类白细胞抗原 基因多态性 

分 类 号：R512.62[医药卫生—内科学]