山羊Apaf-3基因的cDNA克隆、序列分析及组织表达研究  

cDNA cloning,sequence analysis and tissue expression of Apaf-3 gene in goats

作　　者：罗斌[1] 卢建远[1] 马力[1] 胡亮[1] 刘霜[1] 杨珂伟 字向东[1] 

机构地区：[1]西南民族大学国家民委动物科学重点实验室,四川成都610041

出　　处：《西南民族大学学报（自然科学版）》2015年第3期285-290,共6页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：四川省应用基础项目(2013JY0043);西南民族大学研究生创新型科研项目(CX2014SZ71)

摘　　要：对单胎藏山羊和多胎金堂黑山羊Apaf-3基因cDNA克隆、序列分析及组织表达特性研究.采集5只多胎金堂黑山羊和5只单胎藏山羊在发情期的卵巢、垂体等组织样,进行Apaf-3基因的cDNA克隆、序列分析,并采用定量PCR技术对其mRNA进行组织表达量研究.结果,克隆出山羊Apaf-3基因长度为1731bp编码区全长为1245bp,编码414个氨基酸.Apaf-3基因在两种山羊中序列相同,且在5种组织中的表达均无差异.同源性分析表明凋亡基因Apaf-3在动物的进化中保守性低,与山羊多羔性状的相关性需要进一步研究.The objective of this study was to obtain and analyze the sequence of Apaf-3 gene and to reveal the expression of Apaf-3 mRNA in different tissues of non-prolific Tibetan goats and prolific Jintang black goats. Ovaries, pituitaries and other tis- sue samples were collected from five non-prolific Tibetan goats and five prolific Jintang black goats that were in estrus. Apaf-3 eDNA was cloned and analyzed by RT-PCR, and its mRNA expressions in tissues were determined by qPCR. The results showed that the cloned goat Apaf-3 gene was 1731 bp and coding region sequence （CDS） was 1245bp long, encoding 414 amino acids. The results of homology analysis showed that Apaf-3 gene sequence are identical in both breeds. There was no differential expression in five tissues between the two breeds but Apaf-3 in animal evolution is less conservative, and its association with goat prolificacy trait needs to be further studied.

关 键 词：山羊 Apaf-3基因 定量PCR 

分 类 号：S814[农业科学—畜牧学] S827[农业科学—畜牧兽医]