一株短小芽孢杆菌的16S rDNA基因序列分析  

Sequence analysis of 16S rDNA gene of Bacillus pumilus

作　　者：李雪峰[1] 王利[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041

出　　处：《西南民族大学学报（自然科学版）》2015年第3期291-294,共4页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：四川省科技支撑项目(2013FZ0014);四川省科技创新苗子工程项目(2014-127);西南民族大学研究生学位点建设项目(2015XWD-S071007)

摘　　要：从黄颡鱼中分离得到一株优势菌BP-1,经过形态观察、生理生化试验和16S rDNA基因序列分析,表明该菌为发酵型,能运动,革兰氏阳性杆菌.获得的16S rDNA基因序列长度为1478 bp,Gen Bank数据库中登录号为KP299290.该序列与GenBank数据库中多条短小芽孢杆菌16S rDNA基因序列相似性高达99%,系统进化树上与短小芽孢杆菌亲缘关系最近,从而判定菌株BP-1为短小芽孢杆菌.这为短小芽孢杆菌的鉴定和分类提供科学依据.One predominant bacterial strain BP - 1 was isolated from Pelteobagrus fulvidraco. The isolate was identified by the morphology, physical and chemical characters, and 16SrDNA sequence analysis. The results showed that strain BP- 1 was gram positive short rods bacteria,with dynamic and fermentation functions. The length of this 16S rDNA sequence is 1478 bp,whieh shows 99% homology with Bacillus pumilus. This sequence was deposited in GenBank database as KP299290. Its kinship with Bacillus pumilus was the closest on the phylogenetic tree. Thus it was confirmed that strain BP - 1 was Bacillus purnilus. This provides a scientific basis for taxonomic studies of Bacillus pumilus.

关 键 词：短小芽孢杆菌 系统发育 

分 类 号：Q933[生物学—微生物学]