鸡腺胃型传染性支气管炎病毒山东分离株S基因的克隆及序列分析  

Cloning and Sequence Analysis of S Gene of Shandong Isolates of Proventriculus Type Avian Infectious Bronchitis Virus

作　　者：王玉东[1] 刘俊辉[1] 郑增忍[1] 王君玮[1] 王永玲[1] 王晓燕[2] 王树双[1] 牛钟相[3] 

机构地区：[1]中国动物卫生与流行病学中心,山东青岛266032 [2]中国农业大学国际合作与交流处,北京100083 [3]山东农业大学动物科技学院,山东泰安271018

出　　处：《动物医学进展》2015年第6期104-110,共7页Progress In Veterinary Medicine

摘　　要：为研究3株鸡腺胃型传染性支气管炎病毒(IBV)山东分离株(青岛腺胃株QX株、东营腺胃株DY株、肥城腺胃株FC株)病毒纤突蛋白基因(S基因)的核苷酸特征,与呼吸型M41标准株及肾型IBV进行S基因核苷酸进行同源性比较。采用RT-PCR技术方法,对IBV分离株(QX、DY、FC)及标准毒株M41株S基因进行克隆和序列测定,对其中QX和DY株S基因核苷酸分别用3种方法(Clustal V method、Jotun Hein method和Clustal W method)对获得的基因核苷酸序列进行分析。结果显示,QX株和DY株的S基因核苷酸与标准毒株M41株S基因核苷酸的同源性最高为79.3%和79.2%,最小差异性为24.5%和22.3%。QX株和DY株与肾型IB分离株HD同源性最高为78.4%和78.9%,最小差异性均为22.7%。HD株与M41株的同源性最高达到80.8%。通过对S基因进化关系分析,说明腺胃型IBV山东分离株(QX、DY株)与呼吸型和肾型IBV是引起不同组织嗜性的IBV变异株,与呼吸型IBV和肾型IBV有明显的差异,是在IBV遗传变异进化过程中具有重要地位的进化阶段。In order to characterize the nucleotides of spike protein S genes of three Shandong isolates （Qing- dao Strain QX, Dongying Strain DY and Feicheng Strain FC） of proventriculus type avian infectious bron- chitis virus （IBV）, and to compare the homology of their S gene nucleotides with respiratory type IBV M41 and nephric type IBV, RT-PCR was used to clone and sequence the S genes of the IBV isolates （QX, DY and FC） and the reference strain M41. The S genes of QX and DY strains were sequenced by Clustal V method, Jotun Hein method and Clustal W method respectively. The results showed that the highest ho- mology of the S gene nucleotides of Strains QX and DY with the reference strain M41 was up to 79.3 % and 79.2% respectively with the least variation of 24.50% and 22.3 % the highest homology of the S gene nu- cleotides of Strains QX and DY with nephric type IBV Strain HD was up to 78.4% and 78.9%, both with the least variation of 22.7%. The highest homology between Strain HD of nephric type IBV and M41 was up to 80. 8%. Analysis of the S gene evolution relationship indicated that the proventrivulus type IBV Shandong isolates （QX and DY） were variant strains with different tissue tropism and with significant difference from the respiratory type and nephric type IBV, and possessed an important evolution stage.

关 键 词：鸡 传染性支气管炎病毒 腺胃型 S基因 序列分析 

分 类 号：S852.659.1[农业科学—基础兽医学]