福辛普利对2型糖尿病大鼠肝脏氧化应激的影响及肝损伤的保护作用  

Effects of fosinopril on oxidative stress and protective effects on liver in type 2 diabetic rats

作　　者：李忻[1,2] 顾立刚[1] 赵婷婷[2] 张韫[2] 杨鑫[2] 张浩军[2] 严美花[2] 董晞[2] 赵海玲[2] 文玉敏[2] 李平[2] 

机构地区：[1]北京中医药大学,北京100029 [2]中日友好医院,北京100029

出　　处：《中日友好医院学报》2015年第3期173-176,共4页Journal of China-Japan Friendship Hospital

基　　金：国家自然科学基金(No.81173422);国家国际科技合作专项(No.2011DFA31860)

摘　　要：目的:观察福辛普利对2型糖尿病大鼠肝脏氧化应激的影响和对肝脏的保护作用,并探讨其可能的机制。方法:采用小剂量链脲佐菌素(STZ)腹腔注射加高脂饲料喂养方法,建立2型糖尿病并脂肪肝动物模型。将造模成功的大鼠随机分为正常对照组、模型组、福辛普利组和二甲双胍组,比较各组大鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)和甘油三酯(TG)水平,肝组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、一氧化氮(NO)和TG水平,并在光镜下观察各组大鼠肝组织病理形态学改变。结果:与正常组比较,模型组大鼠血清中ALT、AST、TG水平显著升高,肝匀浆中SOD、CAT、GSH-Px水平明显下降,NO和TG水平升高。与模型组相比,福辛普利组和二甲双胍组大鼠血清中ALT、AST、TG水平显著降低,肝组织匀浆中SOD、CAT、GSH-Px水平明显升高,NO、TG水平显著降低。HE染色显示福辛普利组和二甲双胍组大鼠较模型组大鼠肝细胞脂肪变性明显减少,肝细胞排列基本规则。结论:福辛普利可改善2型糖尿病大鼠肝功能,减轻肝细胞脂肪变性,其作用机制可能一定程度上与增强肝组织抗氧化酶活性、清除脂质过氧化物有关。Objective：To investigate the impacts of fosinopril on oxidative stress in diabetic rats and their protective effect of hepatic injury.Methods：Rat model of type 2 diabetes and fatty liver were established by injecting small dose of STZ and feeding high fat diet.All rats were randomly divided into control group,model group,fosinopril group and mefformin group.Serum alanine aminotransferase （ALT）,aspartate aminotransferase （AST）in serum and superoxide dismutase（SOD）,catalase（CAT）,glutathione peroxidase（GSH-Px）,nitric oxide （NO）and triglyceride （TG）in liver were assayed.The liver tissues were taken for histopathological examination. Results：There were significant differences in levels of ALT and AST in serum and SOD,NO and TG in liver homogenate between normal group and model group.These parameters were improved in fosinopril group.HE staining showed that liver steatosis was significantly attenuated in fosinopril group.Conclusion：The fatty liver in type 2 diabetic rats was significantly improved by fosinopril treatment,which probably associated with the response level to oxidative stress in the liver.

关 键 词：福辛普利 2型糖尿病 肝损伤 氧化应激 

分 类 号：R965[医药卫生—药理学]