原料纯度对白及多糖疏水改性反应取代度的影响  

Effect of raw material purity on the degree of substitution of hydrophobically modified Bletilla striata polysaccharide

作　　者：赵雪竹[1,2] 刘洋 王艳萍[2] 刘福强[2] 张琦[2] 刘丹[2] 赵楠[2] 韩丹[2] 赵庆兰[1,2] 

机构地区：[1]延边大学药学院,吉林延吉133000 [2]解放军208医院,吉林长春130062 [3]沈阳军区长春干休所,吉林长春130000

出　　处：《药学实践杂志》2015年第3期221-225,共5页Journal of Pharmaceutical Practice

基　　金：国家自然科学基金项目(81173559)

摘　　要：目的通过纯化反应原料之一胆甾醇琥珀酸酯(CHS),提高疏水改性白及多糖的胆甾醇基取代度。方法利用重结晶法纯化CHS,并采用差示量热扫描法(DSC)及X射线衍射法(XRD)对CHS纯品进行表征;用高效液相色谱法(HPLC)测定纯化前后产品中游离胆甾醇的含量;1 H-NMR法测定CHS纯化前后改性白及多糖的胆甾醇基取代度。结果重结晶后的CHS纯度较高,具有晶体性质,可明显提高改性白及多糖的胆甾醇基取代度。结论纯化方法简便、可行,为提高改性白及多糖胆甾醇基取代度提供研究基础。Objective To improve the degree of substitution of the cholesteryl by purifying cholesteryl succinyl ester （CHS） ,one of the raw materials .Methods CHS was purified by recrystallization ,the pure CHS structure was characterizate by differential scanning calorimetry （DSC） and X ray diffraction （XRD） ,the free cholesterol content of the pure and crude were determinated by high performance liquid chromatography （HPLC） ,the degree of substitution of the cholesteryl was determina-ted before and after the purified by 1 H-NMR .Result CHS was purer after recrystallization ,with property of crystal ,which could significantly improve the degree of substitution of the cholesteryl .Conclusion The method of purification was simple and feasible ,which was the basis of improving hydrophobically modified Bletilla striata polysaccharide′s degree of substitution of the cholesteryl .

关 键 词：原料纯度 胆甾醇琥珀酸酯 取代度 

分 类 号：R94[医药卫生—药剂学]