大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究  

The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina

作　　者：韩燕[1] 闻俊[2] 周婷婷[1,2] 范国荣[2] 

机构地区：[1]福建中医药大学药学院,福建福州350108 [2]第二军医大学药学院药物分析学教研室,上海200433

出　　处：《药学实践杂志》2015年第3期238-241,共4页Journal of Pharmaceutical Practice

基　　金：国家自然科学基金资助项目(30801541)

摘　　要：目的研究大孔树脂分离纯化栀子豉汤有效成分的工艺条件及优化。方法采用紫外分光光度法测定总环烯醚萜苷和总大豆异黄酮的量,以总环烯醚萜苷和总大豆异黄酮的含量作为指标,采用单因素实验考察上样液浓度、上样量、洗脱剂浓度及用量等对分离纯化工艺的影响。结果最佳工艺条件:上样液最佳吸附浓度为0.1g/ml(该浓度以每1ml中所含生药材质量计),上样量与树脂体积比是2∶1,吸附时间2h,依次用1BV(柱体积)水,6BV 20%乙醇洗脱,6BV 60%乙醇洗脱,收集洗脱液。结论该纯化工艺合理、稳定,可推广于工业生产。Objective To optimize separation and purification technology of Zhizichi decoction by macroporous resina . Methods The content of total iridoid glycosides and total isoflavones were determined by ultraviolet spectrophotometry .Tak-ing the content of total iridoid glycosides and total isoflavones as indexes ,the effect of the concentration of sample solution , medicinal herbs on the amount of sample ,concentration and volume of eluent was investigated by single factor test .Results The optimum separation and purification technology was as following ：the concentration of sample solution was 0 .1 g/ml ,the volume ratio of resin to material drug was 2∶1 ,the adsorption time was 2 h ,and the sample was firstly eluted with 1 BV wa-ter ,then 6 BV 20% ethanol and 60% ethanol ,and the eluent was collected .Conclusion This optimized separation and purifi-cation technology was reasonable and stable ,and it could be extended to large-scale production applications .

关 键 词：栀子豉汤 大孔吸附树脂 分离纯化 总环烯醚萜苷 总大豆异黄酮 

分 类 号：R943[医药卫生—药剂学]