针刺后海穴对自闭症大鼠海马NRXN-1、NLGN-3蛋白表达的影响  

Influence of acupuncture at Houhai(GV1) on protein expression of NRXN-1 and NLGN-3 in hippocampus of rats with autism

作　　者：张学君[1] 洪霖[1] 洪钰竺[1] 黄倩茹[1] 吴强[1] 

机构地区：[1]福建中医药大学,福州350122

出　　处：《中华中医药杂志》2015年第6期2107-2110,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金资助项目(No.81202764);福建省教育厅资助项目(No.JK2012022)~~

摘　　要：目的:观察针刺后海穴对自闭症大鼠海马NRXN-1和NLGN-3蛋白表达的影响。方法:24只自闭症大鼠,随机分为模型组、针刺后海穴组、针刺非穴组,每组8只,另取8只大鼠为空白组。针刺后海穴组选取后海穴,直刺0.3寸,行提插手法,160-200次/min,连续针刺30d。干预结束次日,用水迷宫检测大鼠的学习记忆能力;用免疫组织化学技术检测大鼠海马NRXN-1和NLGN-3蛋白表达情况。结果:与模型组比较,针刺后海组平均逃避潜伏期明显缩短(P<0.05),穿越平台次数明显增加(P<0.05),NRXN-1和NLGN-3蛋白阳性细胞灰度值明显降低(P<0.05)。结论:针刺后海穴可能通过调控海马NRXN-1和NLGN-3蛋白的表达来改善自闭症大鼠的学习记忆能力。Objective： To observe the influence of acupuncture at Houhai （GV1） on the protein expression of NRXN- 1 and NLGN-3 in hippocampus of rats with autism. Methods： 24 rats with autism were randomly divided into model group, Houhai （GV1） group and non-point group, and with eight rats in each group. Rats in Houhai （GV1） group received acupuncture at Houhai （GV1） for 30 days with puncture depth of 0.3 inch, and the lifting and thrusting manipulation was also implemented with speed of 160-200 times/min. On the next day after intervention, the learning and memory ability of rats was determined by using water maze. The protein expression of NLGN-1 and NRXN-3 was detected by using immunohistochemical staining. Results： Compared with model group, the average escape latency of rats in Houhai （GV1） group was obviously shortened （P〈0.05）, the times of traversing platform were increased significantly （P〈0.05）, and positive cells grey value of NLGN-1 and NRXN-3 protein was decreased significantly （P〈0.05）. Conclusion： Acupuncture in Houhai （GV1） could improve the learning and memory ability of rats with autism, and its mechanism may be related to the regulation effect on NLGN-1 and NRXN-3 protein expression in hippocampus.

关 键 词：针刺 后海穴 自闭症 NRXN-1 NLGN-3 

分 类 号：R245[医药卫生—针灸推拿学]